Detection of SARS-CoV-2 RNA in Bivalve Mollusks by Droplet Digital RT-PCR (dd RT-PCR).
COVID-19
PCR
SARS-CoV-2
bivalve
shellfish
variant
Journal
International journal of environmental research and public health
ISSN: 1660-4601
Titre abrégé: Int J Environ Res Public Health
Pays: Switzerland
ID NLM: 101238455
Informations de publication
Date de publication:
14 Jan 2022
14 Jan 2022
Historique:
received:
01
12
2021
revised:
07
01
2022
accepted:
10
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
29
1
2022
Statut:
epublish
Résumé
Bivalve shellfish are readily contaminated by human pathogens present in waters impacted by municipal sewage, and the detection of SARS-CoV-2 in feces of infected patients and in wastewater has drawn attention to the possible presence of the virus in bivalves. The aim of this study was to collect data on SARS-CoV-2 prevalence in bivalve mollusks from harvesting areas of Campania region. A total of 179 samples were collected between September 2019 and April 2021 and were tested using droplet digital RT-PCR (dd RT-PCR) and real-time RT-PCR. Combining results obtained with different assays, SARS-CoV-2 presence was detected in 27/179 (15.1%) of samples. A median viral concentration of 1.1 × 10
Identifiants
pubmed: 35055765
pii: ijerph19020943
doi: 10.3390/ijerph19020943
pmc: PMC8776039
pii:
doi:
Substances chimiques
RNA, Viral
0
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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