Capture and Ex-Situ Analysis of Environmental Biofilms in Livestock Buildings.
biofilm
diversity
livestock building
sampling
surfaces
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
21 Dec 2021
21 Dec 2021
Historique:
received:
30
11
2021
revised:
17
12
2021
accepted:
19
12
2021
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
22
1
2022
Statut:
epublish
Résumé
Little information about biofilm microbial communities on the surface of livestock buildings is available yet. While these spatially organized communities proliferate in close contact with animals and can harbor undesirable microorganisms, no standardized methods have been described to sample them non-destructively. We propose a reproducible coupon-based capture method associated with a set of complementary ex-situ analysis tools to describe the major features of those communities. To demonstrate the biofilm dynamics in a pig farm building, we analyzed the coupons on polymeric and metallic materials, as representative of these environments, over 4 weeks. Confocal laser scanning microscopy (CLSM) revealed a rapid coverage of the coupons with a thick layer of biological material and the existence of dispersed clusters of active metabolic microorganisms. After detaching the cells from the coupons, counts to quantify the CFU/cm
Identifiants
pubmed: 35056451
pii: microorganisms10010002
doi: 10.3390/microorganisms10010002
pmc: PMC8777997
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Association Nationale de la Recherche et de la Technologie
ID : 2020/0548
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