Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
20 01 2022
20 01 2022
Historique:
received:
26
06
2021
accepted:
27
12
2021
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
25
3
2022
Statut:
epublish
Résumé
DNA transfection is an important technology in life sciences, wherein nuclear entry of DNA is necessary to express exogenous DNA. Non-viral vectors and their transfection reagents are useful as safe transfection tools. However, they have no effect on the transfection of non-proliferating cells, the reason for which is not well understood. This study elucidates the mechanism through which transfected DNA enters the nucleus for gene expression. To monitor the behavior of transfected DNA, we introduce plasmid bearing lacO repeats and RFP-coding sequences into cells expressing GFP-LacI and observe plasmid behavior and RFP expression in living cells. RFP expression appears only after mitosis. Electron microscopy reveals that plasmids are wrapped with nuclear envelope (NE)‒like membranes or associated with chromosomes at telophase. The depletion of BAF, which is involved in NE reformation, delays plasmid RFP expression. These results suggest that transfected DNA is incorporated into the nucleus during NE reformation at telophase.
Identifiants
pubmed: 35058555
doi: 10.1038/s42003-022-03021-8
pii: 10.1038/s42003-022-03021-8
pmc: PMC8776997
doi:
Substances chimiques
LEMD2 protein, human
0
Membrane Proteins
0
Nuclear Proteins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
78Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP17K19505
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP18H05528
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP19K06611
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP19H05795
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP19H03394
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP16H06280
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP17H06167
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP15H05972
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP20H00454
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP18H05533
Organisme : MEXT | JST | Core Research for Evolutional Science and Technology (CREST)
ID : JPMJCR15G2
Informations de copyright
© 2022. The Author(s).
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