Tumor radioresistance caused by radiation-induced changes of stem-like cell content and sub-lethal damage repair capability.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 01 2022
20 01 2022
Historique:
received:
08
11
2021
accepted:
07
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
9
3
2022
Statut:
epublish
Résumé
Cancer stem-like cells (CSCs) within solid tumors exhibit radioresistance, leading to recurrence and distant metastasis after radiotherapy. To experimentally study the characteristics of CSCs, radioresistant cell lines were successfully established using fractionated X-ray irradiation. The fundamental characteristics of CSCs in vitro have been previously reported; however, the relationship between CSC and acquired radioresistance remains uncertain. To efficiently study this relationship, we performed both in vitro experiments and theoretical analysis using a cell-killing model. Four types of human oral squamous carcinoma cell lines, non-radioresistant cell lines (SAS and HSC2), and radioresistant cell lines (SAS-R and HSC2-R), were used to measure the surviving fraction after single-dose irradiation, split-dose irradiation, and multi-fractionated irradiation. The SAS-R and HSC2-R cell lines were more positive for one of the CSC marker aldehyde dehydrogenase activity than the corresponding non-radioresistant cell lines. The theoretical model analysis showed that changes in both the experimental-based ALDH (+) fractions and DNA repair efficiency of ALDH (-) fractions (i.e., sub-lethal damage repair) are required to reproduce the measured cell survival data of non-radioresistant and radioresistant cell lines. These results suggest that the enhanced cell recovery in SAS-R and HSC2-R is important when predicting tumor control probability in radiotherapy to require a long dose-delivery time; in other words, intensity-modulated radiation therapy is ideal. This work provides a precise understanding of the mechanism of radioresistance, which is induced after irradiation of cancer cells.
Identifiants
pubmed: 35058559
doi: 10.1038/s41598-022-05172-4
pii: 10.1038/s41598-022-05172-4
pmc: PMC8776741
doi:
Substances chimiques
Aldehyde Dehydrogenase
EC 1.2.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1056Subventions
Organisme : Japan Society for the Promotion of Science
ID : 20K16814
Organisme : Japan Society for the Promotion of Science
ID : 19K17215
Organisme : Japan Society for the Promotion of Science
ID : 19K08141
Informations de copyright
© 2022. The Author(s).
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