An endosome-associated actin network involved in directed apical plasma membrane growth.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 03 2022
07 03 2022
Historique:
received:
13
07
2021
revised:
06
12
2021
accepted:
04
01
2022
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
17
2
2022
Statut:
ppublish
Résumé
Membrane trafficking plays many roles in morphogenesis, from bulk membrane provision to targeted delivery of proteins and other cargos. In tracheal terminal cells of the Drosophila respiratory system, transport through late endosomes balances membrane delivery between the basal plasma membrane and the apical membrane, which forms a subcellular tube, but it has been unclear how the direction of growth of the subcellular tube with the overall cell growth is coordinated. We show here that endosomes also organize F-actin. Actin assembles around late endocytic vesicles in the growth cone of the cell, reaching from the tip of the subcellular tube to the leading filopodia of the basal membrane. Preventing nucleation of endosomal actin disturbs the directionality of tube growth, uncoupling it from the direction of cell elongation. Severing actin in this area affects tube integrity. Our findings show a new role for late endosomes in directing morphogenesis by organizing actin, in addition to their known role in membrane and protein trafficking.
Identifiants
pubmed: 35061016
pii: 212975
doi: 10.1083/jcb.202106124
pmc: PMC8789128
pii:
doi:
Substances chimiques
Actins
0
Drosophila Proteins
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Marie Curie
Pays : United Kingdom
Informations de copyright
© 2022 Ríos-Barrera and Leptin.
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