A Cross Sectional Sampling Reveals Novel Coronaviruses in Bat Populations of Georgia.
alphacoronavirus
bats
betacoronavirus
coronavirus
eastern europe
georgia
phylogeny
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
31 12 2021
31 12 2021
Historique:
received:
13
11
2021
revised:
17
12
2021
accepted:
18
12
2021
entrez:
22
1
2022
pubmed:
23
1
2022
medline:
4
2
2022
Statut:
epublish
Résumé
Mammal-associated coronaviruses have a long evolutionary history across global bat populations, which makes them prone to be the most likely ancestral origins of coronavirus-associated epidemics and pandemics globally. Limited coronavirus research has occurred at the junction of Europe and Asia, thereby investigations in Georgia are critical to complete the coronavirus diversity map in the region. We conducted a cross-sectional coronavirus survey in bat populations at eight locations of Georgia, from July to October of 2014. We tested 188 anal swab samples, remains of previous pathogen discovery studies, for the presence of coronaviruses using end-point pan-coronavirus RT-PCR assays. Samples positive for a 440 bp amplicon were Sanger sequenced to infer coronavirus subgenus or species through phylogenetic reconstructions. Overall, we found a 24.5% positive rate, with 10.1% for
Identifiants
pubmed: 35062276
pii: v14010072
doi: 10.3390/v14010072
pmc: PMC8778869
pii:
doi:
Substances chimiques
RNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, U.S. Gov't, P.H.S.
Langues
eng
Sous-ensembles de citation
IM
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