Human endothelial cells in high glucose: New clues from culture in 3D microfluidic chips.
Apoptosis
/ physiology
Cell Culture Techniques, Three Dimensional
/ methods
Cells, Cultured
Cytoskeleton
/ metabolism
Endothelium, Vascular
/ metabolism
Glucose
/ metabolism
Glycocalyx
/ metabolism
Human Umbilical Vein Endothelial Cells
/ metabolism
Humans
Microfluidics
/ methods
Microvessels
/ metabolism
Stress, Mechanical
cytoskeleton
diabetes
endothelium
glucose
microfluidics
microvasculature on a chip
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
02 2022
02 2022
Historique:
revised:
12
12
2021
received:
02
06
2021
accepted:
17
12
2021
entrez:
23
1
2022
pubmed:
24
1
2022
medline:
15
2
2022
Statut:
ppublish
Résumé
Several studies have demonstrated the role of high glucose in promoting endothelial dysfunction utilizing traditional two-dimensional (2D) culture systems, which, however, do not replicate the complex organization of the endothelium within a vessel constantly exposed to flow. Here we describe the response to high glucose of micro- and macro-vascular human endothelial cells (EC) cultured in biomimetic microchannels fabricated through soft lithography and perfused to generate shear stress. In 3D macrovascular EC exposed to a shear stress of 0.4 Pa respond to high glucose with cytoskeletal remodeling and alterations in cell shape. Under the same experimental conditions, these effects are more pronounced in microvascular cells that show massive cytoskeletal disassembly and apoptosis after culture in high glucose. However, when exposed to a shear stress of 4 Pa, which is physiological in the microvasculature, human dermal microvascular endothelial cells (HDMEC) show alterations of the cytoskeleton but no apoptosis. This result emphasizes the sensitivity of HDMEC to different regimens of flow. No significant variations in the thickness of glycocalyx were detected in both human endothelial cells from the umbilical vein and HDMEC exposed to high glucose in 3D, whereas clear differences emerge between cells cultured in static 2D versus microfluidic channels. We conclude that culture in microfluidic microchannels unveils unique insights into endothelial dysfunction by high glucose.
Identifiants
pubmed: 35066939
doi: 10.1096/fj.202100914R
doi:
Substances chimiques
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e22137Informations de copyright
© 2022 Federation of American Societies for Experimental Biology.
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