Herpes simplex virus-1 utilizes the host actin cytoskeleton for its release from axonal growth cones.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
20
09
2021
accepted:
10
01
2022
revised:
03
02
2022
pubmed:
25
1
2022
medline:
23
2
2022
entrez:
24
1
2022
Statut:
epublish
Résumé
Herpes simplex virus type 1 (HSV-1) has evolved mechanisms to exploit the host cytoskeleton during entry, replication and exit from cells. In this study, we determined the role of actin and the molecular motor proteins, myosin II and myosin V, in the transport and release of HSV-1 from axon termini, or growth cones. Using compartmentalized neuronal devices, we showed that inhibition of actin polymerization, but not actin branching, significantly reduced the release of HSV-1 from axons. Furthermore, we showed that inhibition of myosin V, but not myosin II, also significantly reduced the release of HSV-1 from axons. Using confocal and electron microscopy, we determined that viral components are transported along axons to growth cones, despite actin or myosin inhibition. Overall, our study supports the role of actin in virus release from axonal growth cones and suggests myosin V as a likely candidate involved in this process.
Identifiants
pubmed: 35073379
doi: 10.1371/journal.ppat.1010264
pii: PPATHOGENS-D-21-01923
pmc: PMC8812851
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010264Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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