Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 01 2022
24 01 2022
Historique:
received:
26
11
2021
accepted:
11
01
2022
entrez:
25
1
2022
pubmed:
26
1
2022
medline:
5
3
2022
Statut:
epublish
Résumé
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
Identifiants
pubmed: 35075208
doi: 10.1038/s41598-022-05315-7
pii: 10.1038/s41598-022-05315-7
pmc: PMC8786948
doi:
Types de publication
Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1237Subventions
Organisme : Health Research Fund (Instituto de Salud Carlos III. Plan Nacional de I+D+I 2013-2016)
ID : PI18/01068
Organisme : Health Research Fund (Instituto de Salud Carlos III. Plan Nacional de I+D+I 2013-2016)
ID : PI18/00997
Organisme : Instituto de Salud Carlos III
ID : PI15/01073
Informations de copyright
© 2022. The Author(s).
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