Imaging Keratan Sulfate in Ocular Tissue Sections by Immunofluorescence Microscopy and LA-ICP-MS.

Animals Antibodies, Monoclonal Cornea / diagnostic imaging Glycosaminoglycans Keratan Sulfate Microscopy, Fluorescence Rats
ICP-MS cornea extracellular matrix glycosaminoglycans imaging immunofluorescence immunohistochemistry keratan sulfate

Journal

ACS applied bio materials
ISSN: 2576-6422
Titre abrégé: ACS Appl Bio Mater
Pays: United States
ID NLM: 101729147

Informations de publication

Date de publication:
21 02 2022
Historique:
pubmed: 26 1 2022
medline: 9 4 2022
entrez: 25 1 2022
Statut: ppublish

Résumé

Carbohydrate-specific antibodies can serve as valuable tools to monitor alterations in the extracellular matrix resulting from pathologies. Here, the keratan sulfate-specific monoclonal antibody MZ15 was characterized in more detail by immunofluorescence microscopy as well as laser ablation ICP-MS using tissue cryosections and paraffin-embedded samples. Pretreatment with keratanase II prevented staining of samples and therefore demonstrated efficient enzymatic keratan sulfate degradation. Random fluorescent labeling and site-directed introduction of a metal cage into MZ15 were successful and allowed for a highly sensitive detection of the keratan sulfate landscape in the corneal stroma from rats and human tissue.

Identifiants

DOI: 10.1021/acsabm.1c01240 PMID: 35076201
pubmed: 35076201
doi: 10.1021/acsabm.1c01240
doi:

Substances chimiques

Antibodies, Monoclonal 0
Glycosaminoglycans 0
Keratan Sulfate 9056-36-4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

853-861

Auteurs

Burak Boyraz (B)

Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Augustenburger Platz 1, Berlin 13353, Germany.
Freie Universität Berlin, Fachbereich Biologie, Chemie, Pharmazie, Arnimallee 22, Berlin 14195, Germany.
ORCID: 0000-0002-1445-6282

Jessica Saatz (J)

Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Strasse, 11, Berlin 12489, Germany.

Inga-Marie Pompös (IM)

Klinik für Augenheilkunde, Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Augustenburger Platz 1, Berlin 13353, Germany.
ORCID: 0000-0001-9314-8378

Michel Gad (M)

Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Strasse, 11, Berlin 12489, Germany.
Department Chemie und Biologie, Universität Siegen, Adolf-Reichwein-Strasse 2, Siegen 57076, Germany.
ORCID: 0000-0003-0340-8426

Jens Dernedde (J)

Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Augustenburger Platz 1, Berlin 13353, Germany.
ORCID: 0000-0001-5319-7677

Anna-Karina B Maier (AB)

Klinik für Augenheilkunde, Campus Virchow-Klinikum, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Augustenburger Platz 1, Berlin 13353, Germany.

Oren Moscovitz (O)

Biomolecular Systems Department, Max-Planck-Institute for Colloids and Interfaces, Am Mühlenberg 1, Potsdam 14476, Germany.

Peter H Seeberger (PH)

Biomolecular Systems Department, Max-Planck-Institute for Colloids and Interfaces, Am Mühlenberg 1, Potsdam 14476, Germany.

Heike Traub (H)

Bundesanstalt für Materialforschung und -prüfung (BAM), Richard-Willstätter-Strasse, 11, Berlin 12489, Germany.

Rudolf Tauber (R)

Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, Augustenburger Platz 1, Berlin 13353, Germany.
ORCID: 0000-0002-0687-3940

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Imaging Keratan Sulfate in Ocular Tissue...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Imaging Keratan Sulfate in Ocular Tissue...
questionsmedicales.fr › Organes des sens › Oeil › Pôle antérieur du bulbe oculaire › Cornée › Imaging Keratan Sulfate in Ocular Tissue...
questionsmedicales.fr › Glucides › Polyosides › Glycosaminoglycanes › Imaging Keratan Sulfate in Ocular Tissue...
questionsmedicales.fr › Glucides › Polyosides › Glycosaminoglycanes › Kératane sulfate › Imaging Keratan Sulfate in Ocular Tissue...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Imaging Keratan Sulfate in Ocular Tissue...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Imaging Keratan Sulfate in Ocular Tissue...