Modular assembly of the principal microtubule nucleator γ-TuRC.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 01 2022
25 01 2022
Historique:
received:
28
08
2021
accepted:
07
01
2022
entrez:
26
1
2022
pubmed:
27
1
2022
medline:
17
2
2022
Statut:
epublish
Résumé
The gamma-tubulin ring complex (γ-TuRC) is the principal microtubule nucleation template in vertebrates. Recent cryo-EM reconstructions visualized the intricate quaternary structure of the γ-TuRC, containing more than thirty subunits, raising fundamental questions about γ-TuRC assembly and the role of actin as an integral part of the complex. Here, we reveal the structural mechanism underlying modular γ-TuRC assembly and identify a functional role of actin in microtubule nucleation. During γ-TuRC assembly, a GCP6-stabilized core comprising GCP2-3-4-5-4-6 is expanded by stepwise recruitment, selective stabilization and conformational locking of four pre-formed GCP2-GCP3 units. Formation of the lumenal bridge specifies incorporation of the terminal GCP2-GCP3 unit and thereby leads to closure of the γ-TuRC ring in a left-handed spiral configuration. Actin incorporation into the complex is not relevant for γ-TuRC assembly and structural integrity, but determines γ-TuRC geometry and is required for efficient microtubule nucleation and mitotic chromosome alignment in vivo.
Identifiants
pubmed: 35078983
doi: 10.1038/s41467-022-28079-0
pii: 10.1038/s41467-022-28079-0
pmc: PMC8789826
doi:
Substances chimiques
Actins
0
Microtubule-Associated Proteins
0
Recombinant Proteins
0
TUBGCP6 protein, human
0
Tubulin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
473Informations de copyright
© 2022. The Author(s).
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