Aptamer based point of care diagnostic for the detection of food allergens.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 01 2022
25 01 2022
Historique:
received:
08
11
2021
accepted:
07
01
2022
entrez:
26
1
2022
pubmed:
27
1
2022
medline:
5
3
2022
Statut:
epublish
Résumé
Aptamers, due to their small size, strong target affinity, and ease of chemical modification, are ideally suited for molecular detection technologies. Here, we describe successful use of aptamer technology in a consumer device for the detection of peanut antigen in food. The novel aptamer-based protein detection method is robust across a wide variety of food matrices and sensitive to peanut protein at concentrations as low as 12.5 ppm (37.5 µg peanut protein in the sample). Integration of the assay into a sensitive, stable, and consumer friendly portable device will empower users to easily and quickly assess the presence of peanut allergens in foods before eating. With many food reactions occurring outside the home, the type of technology described here has significant potential to improve lives for children and families.
Identifiants
pubmed: 35079047
doi: 10.1038/s41598-022-05265-0
pii: 10.1038/s41598-022-05265-0
pmc: PMC8789827
doi:
Substances chimiques
Allergens
0
Antigens, Plant
0
Aptamers, Nucleotide
0
Ara h 1 protein, Arachis hypogaea
0
Membrane Proteins
0
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1303Informations de copyright
© 2022. The Author(s).
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