Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
27 01 2022
27 01 2022
Historique:
received:
03
12
2020
accepted:
04
01
2022
entrez:
28
1
2022
pubmed:
29
1
2022
medline:
19
2
2022
Statut:
epublish
Résumé
Diffraction-limited two-photon microscopy permits minimally invasive optical monitoring of neuronal activity. However, most conventional two-photon microscopes impose significant constraints on the size of the imaging field-of-view and the specific shape of the effective excitation volume, thus limiting the scope of biological questions that can be addressed and the information obtainable. Here, employing a non-telecentric optical design, we present a low-cost, easily implemented and flexible solution to address these limitations, offering a several-fold expanded three-dimensional field of view. Moreover, rapid laser-focus control via an electrically tunable lens allows near-simultaneous imaging of remote regions separated in three dimensions and permits the bending of imaging planes to follow natural curvatures in biological structures. Crucially, our core design is readily implemented (and reversed) within a matter of hours, making it highly suitable as a base platform for further development. We demonstrate the application of our system for imaging neuronal activity in a variety of examples in zebrafish, mice and fruit flies.
Identifiants
pubmed: 35087041
doi: 10.1038/s41467-022-28192-0
pii: 10.1038/s41467-022-28192-0
pmc: PMC8795402
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
544Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R014817/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S00310X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P006639/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P010121/1
Pays : United Kingdom
Organisme : Wellcome Trust (Wellcome)
ID : 220277/Z20/Z
Organisme : Medical Research Council
ID : MC_PC_15071
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
Références
Nat Methods. 2010 May;7(5):399-405
pubmed: 20400966
Nat Methods. 2011 Feb;8(2):139-42
pubmed: 21217749
Nat Methods. 2005 Dec;2(12):932-40
pubmed: 16299478
J Microsc. 2011 May;242(2):157-65
pubmed: 21118240
Pflugers Arch. 2009 Apr;457(6):1393-414
pubmed: 19023590
Nature. 2017 Feb 23;542(7642):439-444
pubmed: 28178238
Neuron. 2006 Jun 15;50(6):823-39
pubmed: 16772166
Cell. 2019 May 2;177(4):1050-1066.e14
pubmed: 30982596
Elife. 2016 Jun 14;5:
pubmed: 27300105
Microsc Res Tech. 1999 Nov 1;47(3):206-9
pubmed: 10544335
J Biomed Opt. 2001 Jul;6(3):266-72
pubmed: 11516315
Biophys J. 2000 Apr;78(4):2159-62
pubmed: 10733993
Biophys J. 2008 Dec 15;95(12):6025-43
pubmed: 18835896
Nat Methods. 2016 Dec;13(12):1021-1028
pubmed: 27798612
Elife. 2017 Aug 16;6:
pubmed: 28812976
Annu Rev Neurosci. 2019 Jul 8;42:295-313
pubmed: 31283896
Nat Biotechnol. 2003 Nov;21(11):1369-77
pubmed: 14595365
PLoS Biol. 2012;10(10):e1001400
pubmed: 23055825
Biomed Opt Express. 2014 Apr 18;5(5):1588-609
pubmed: 24877017
Opt Lett. 2016 Mar 1;41(5):855-8
pubmed: 26974063
J Neurosci Methods. 2021 Jul 1;358:109173
pubmed: 33839190
Nat Methods. 2017 Apr;14(4):420-426
pubmed: 28319111
Microsc Res Tech. 2004 Dec;65(6):308-14
pubmed: 15662621
Opt Lett. 1995 May 15;20(10):1213-5
pubmed: 19859476
Front Neural Circuits. 2013 Apr 24;7:76
pubmed: 23630470
Neuron. 2020 Nov 25;108(4):722-734.e5
pubmed: 32966764
Opt Express. 2015 Jun 1;23(11):13833-47
pubmed: 26072755
Nat Biotechnol. 2016 Aug;34(8):857-62
pubmed: 27347754
Annu Rev Neurosci. 2018 Jul 8;41:431-452
pubmed: 29709208
Nat Methods. 2014 Sep;11(9):883-4
pubmed: 25068735
Dev Dyn. 2007 Nov;236(11):3088-99
pubmed: 17937395
Sci Rep. 2020 Mar 10;10(1):4399
pubmed: 32157103
PLoS One. 2014 Sep 24;9(9):e108697
pubmed: 25250714
Biomed Opt Express. 2018 Dec 19;10(1):267-282
pubmed: 30775099
Nat Commun. 2021 Nov 17;12(1):6639
pubmed: 34789723
PLoS Biol. 2020 Apr 20;18(4):e3000701
pubmed: 32310946
Biomed Opt Express. 2011 Jul 1;2(7):2035-46
pubmed: 21750778
Science. 1990 Apr 6;248(4951):73-6
pubmed: 2321027
Biomed Opt Express. 2015 Jun 17;6(7):2575-87
pubmed: 26203383
Nat Methods. 2013 Oct;10(10):1013-20
pubmed: 24013820
Opt Express. 2013 Mar 11;21(5):5998-6008
pubmed: 23482168
Front Neuroanat. 2019 Feb 08;13:7
pubmed: 30800060
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
Cereb Cortex. 2005 May;15(5):639-45
pubmed: 15342433
Cell Rep. 2019 May 14;27(7):2229-2240.e4
pubmed: 31091458
Nat Methods. 2013 May;10(5):413-20
pubmed: 23524393
J Neurophysiol. 2016 Sep 1;116(3):1012-23
pubmed: 27281749
Biophys J. 2001 Apr;80(4):2029-36
pubmed: 11259316
Nat Neurosci. 2017 Apr;20(4):620-628
pubmed: 28250408
Neuron. 2021 Jun 2;109(11):1810-1824.e9
pubmed: 33878295
Curr Biol. 2018 Jul 9;28(13):2018-2032.e5
pubmed: 29937350
Nature. 2015 Jan 8;517(7533):200-4
pubmed: 25363782
Cell Rep. 2020 Feb 11;30(6):2006-2017.e3
pubmed: 32049027
Nat Commun. 2016 Feb 03;7:10590
pubmed: 26838932
Development. 2009 Jun;136(12):1963-75
pubmed: 19465594
Development. 2014 Mar;141(5):1167-74
pubmed: 24550120
Development. 2008 Sep;135(17):2865-71
pubmed: 18653554
Neuron. 1997 Mar;18(3):351-7
pubmed: 9115730
Biophys J. 1999 May;76(5):2412-20
pubmed: 10233058
Sci Rep. 2018 Jun 18;8(1):9252
pubmed: 29915203
Neurophotonics. 2018 Apr;5(2):025001
pubmed: 29487876
Elife. 2018 Feb 07;7:
pubmed: 29412138