Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging.
aging
hippocampus
human
mouse
neurogenesis
neuroscience
regenerative medicine
stem cell
stem cells
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
28 01 2022
28 01 2022
Historique:
received:
01
03
2021
accepted:
12
10
2021
entrez:
28
1
2022
pubmed:
29
1
2022
medline:
23
2
2022
Statut:
epublish
Résumé
Advancing age causes reduced hippocampal neurogenesis, associated with age-related cognitive decline. The spatial relationship of age-induced alterations in neural stem cells (NSCs) and surrounding cells within the hippocampal niche remains poorly understood due to limitations of antibody-based cellular phenotyping. We established iterative indirect immunofluorescence imaging (4i) in tissue sections, allowing for simultaneous detection of 18 proteins to characterize NSCs and surrounding cells in 2-, 6-, and 12-month-old mice. We show that reorganization of the dentate gyrus (DG) niche already occurs in middle-aged mice, paralleling the decline in neurogenesis. 4i-based tissue analysis of the DG identifies changes in cell-type contributions to the blood-brain barrier and microenvironments surrounding NSCs to play a pivotal role to preserve neurogenic permissiveness. The data provided represent a resource to characterize the principles causing alterations of stem cell-associated plasticity within the aging DG and provide a blueprint to analyze somatic stem cell niches across lifespan in complex tissues.
Identifiants
pubmed: 35089129
doi: 10.7554/eLife.68000
pii: 68000
pmc: PMC8798039
doi:
pii:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, Cole et al.
Déclaration de conflit d'intérêts
JC, JS, DG, SJ No competing interests declared, GG, LP Inventor of a patent related to 4i technology (WO2019207004A1)
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