Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods.

Animals Melanoma / metabolism Proteins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods Swine Swine, Miniature Tandem Mass Spectrometry
Mass spectrometry MeLiM Melanoma

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
01 Apr 2022
Historique:
received: 10 10 2021
revised: 19 01 2022
accepted: 21 01 2022
pubmed: 30 1 2022
medline: 1 4 2022
entrez: 29 1 2022
Statut: ppublish

Résumé

Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.

Identifiants

DOI: 10.1016/j.ijbiomac.2022.01.139 PMID: 35090942
pubmed: 35090942
pii: S0141-8130(22)00154-4
doi: 10.1016/j.ijbiomac.2022.01.139
pii:
doi:

Substances chimiques

Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

583-592

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Lucie Vaníčková (L)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic; Department of Forest Botany, Dendrology and Geobiocenology, Faculty of Forest and Wood Technology, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic. Electronic address: lucie.vanickova@mendelu.cz.

Tomáš Do (T)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic.

Markéta Vejvodová (M)

Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech Republic.

Vratislav Horák (V)

Institute of Animal Physiology and Genetics, Laboratory of Applied Proteome Analyses, Czech Academy of Sciences, v.v.i., Rumburska 89, CZ-27721 Libechov, Czech Republic.

Martin Hubálek (M)

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, v.v.i., Flemingovo nam. 542/2, CZ-16000 Prague, Czech Republic.

Gabriella Emri (G)

Department of Dermatology, Faculty of Medicine, University of Debrecen, Egyetem ter 1, HU-4031 Debrecen, Hungary.

Kristýna Zemánková (K)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic.

Kristýna Pavelicová (K)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic.

Soňa Křížková (S)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic.

Veronika Faltusová (V)

Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech Republic.

Antonio Pompeiano (A)

Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic; Department of Forest Botany, Dendrology and Geobiocenology, Faculty of Forest and Wood Technology, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic.

Markéta Vaculovičová (M)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic.

Ondřej Zítka (O)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic.

Tomáš Vaculovič (T)

Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 753/5, CZ-625 00 Brno, Czech Republic; Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynska dolina, Ilkovicova 6, SK-84215 Bratislava, Slovakia.

Vojtěch Adam (V)

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1665/1, CZ-61300 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, CZ-61200 Brno, Czech Republic.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Mapping of MeLiM melanoma combining ICP-MS and...
questionsmedicales.fr › Tumeurs › Tumeurs par type histologique › Naevus et mélanomes › Mélanome › Mapping of MeLiM melanoma combining ICP-MS and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Mapping of MeLiM melanoma combining ICP-MS and...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Mapping of MeLiM melanoma combining ICP-MS and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Mapping of MeLiM melanoma combining ICP-MS and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Sus scrofa › Porc miniature › Mapping of MeLiM melanoma combining ICP-MS and...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Mapping of MeLiM melanoma combining ICP-MS and...