Establishment of an antigen-capture enzyme-linked immunosorbent assay for detecting avian reticuloendotheliosis virus.

Animals Antibodies, Viral Chickens Enzyme-Linked Immunosorbent Assay / methods Mice Poultry Diseases Rabbits Reticuloendotheliosis Viruses, Avian Reticuloendotheliosis virus
Antigen-capture ELISA Avian reticuloendotheliosis virus Polyclonal antibody gp90

Journal

Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839

Informations de publication

Date de publication:
04 2022
Historique:
received: 08 12 2021
revised: 19 01 2022
accepted: 21 01 2022
pubmed: 30 1 2022
medline: 23 3 2022
entrez: 29 1 2022
Statut: ppublish

Résumé

In this study, an antigen-capturing enzyme-linked immunosorbent assay (AC-ELISA) was established for the detection of avian reticuloendotheliosis virus (REV) using monoclonal and polyclonal antibodies against gp90. New Zealand white rabbits were immunized with recombinant REV-gp90 protein, and polyclonal antibodies were obtained after purification and used as the capture antibody. Mice monoclonal antibody 1A12D against REV-gp90 protein previously prepared in our laboratory was used as the detection antibody. The specificity of the AC-ELISA was confirmed with REV, avian leukosis virus subgroup J, Marek's disease virus serotype Ⅰ, avian hepatitis E virus and Fowl adenovirus serotype 4. The results showed that the AC-ELISA had specific binding reaction with REV, and did not react with other viruses. The detection limit of this assay was 195 TCID

Identifiants

DOI: 10.1016/j.jviromet.2022.114476 PMID: 35090953
pubmed: 35090953
pii: S0166-0934(22)00023-4
doi: 10.1016/j.jviromet.2022.114476
pii:
doi:

Substances chimiques

Antibodies, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

114476

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Xinyuan Miao (X)

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.

Qun Wang (Q)

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.

Yawen Zhang (Y)

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.

Shuang Chang (S)

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China.

Peng Zhao (P)

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China. Electronic address: zhaopeng@sdau.edu.cn.

Yixin Wang (Y)

College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, Shandong, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, Shandong, China. Electronic address: sdauwyx@163.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Establishment of an antigen-capture...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Establishment of an antigen-capture...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › Establishment of an antigen-capture...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Establishment of an antigen-capture...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Establishment of an antigen-capture...
questionsmedicales.fr › Maladies de l'animal › Maladies des oiseaux › Maladies de la volaille › Establishment of an antigen-capture...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › Establishment of an antigen-capture...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Gammaretrovirus › Virus de la réticuloendothéliose aviaire › Establishment of an antigen-capture...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Gammaretrovirus › Virus de la réticuloendothéliose aviaire › Virus de la réticuloendothéliose › Establishment of an antigen-capture...