Human placental mesenchymal stromal cell therapy restores the cytokine efflux and insulin signaling in the skeletal muscle of obesity-induced type 2 diabetes rat model.
Cytokines
Glut4
Human placental mesenchymal stromal cells
Insulin signaling
Skeletal muscle tissue
WNIN/GR-Ob rats
Journal
Human cell
ISSN: 1749-0774
Titre abrégé: Hum Cell
Pays: Japan
ID NLM: 8912329
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
01
07
2021
accepted:
14
12
2021
pubmed:
30
1
2022
medline:
26
2
2022
entrez:
29
1
2022
Statut:
ppublish
Résumé
Obesity poses a significant risk factor for the onset of metabolic syndrome with allied complications, wherein mesenchymal stem cell therapy is seen as a promising treatment for obesity-induced metabolic syndrome. In the present study, we aim to explore the beneficial effects of the human placental mesenchymal stromal cells (P-MSCs) on obesity-associated insulin resistance (IR) including inflammation. To understand this, we have analyzed the peripheral blood glucose, serum insulin levels by ELISA, and the glucose uptake capacity of skeletal muscle by a 2-NBDG assay using flow cytometry in WNIN/GR-Ob rats treated with and without P-MSCs. Also, we have studied insulin signaling and cytokine profile in the skeletal muscle by western blotting, dot blotting, and Multiplex-ELISA techniques. The skeletal muscle of WNIN/GR-Ob rats demonstrates dysregulation of cytokines, altered glucose uptake vis-a-vis insulin signaling. However, P-MSCs' treatment was effective in WNIN/GR-Ob rats as compared to its control, to restore HOMA-IR, re-establishes dysregulated cytokines and PI3K-Akt pathway in addition to enhanced Glut4 expression and glucose uptake studied in skeletal muscle. Overall, our data advocate the beneficial effects of P-MSCs to ameliorate inflammatory milieu, improve insulin sensitivity, and normalize glucose homeostasis underlining the Ob-T2D conditions, and we attribute for immunomodulatory, paracrine, autocrine, and multipotent functions of P-MSCs.
Identifiants
pubmed: 35091972
doi: 10.1007/s13577-021-00664-3
pii: 10.1007/s13577-021-00664-3
doi:
Substances chimiques
Cytokines
0
Insulin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
557-571Subventions
Organisme : indian council of medical research
ID : 15-BS03
Organisme : indian council of medical research
ID : 18-BS10
Organisme : indian council of medical research
ID : 5/3/8/31/ITR-F/2018-ITR
Informations de copyright
© 2021. The Author(s) under exclusive licence to Japan Human Cell Society.
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