Differences in microbial profile of endometrial fluid and tissue samples in women with in vitro fertilization failure are driven by Lactobacillus abundance.
endometrial microbiome
endometrial microbiota
implantation failure
in vitro fertilization
infertility
uterine microbiota
Journal
Acta obstetricia et gynecologica Scandinavica
ISSN: 1600-0412
Titre abrégé: Acta Obstet Gynecol Scand
Pays: United States
ID NLM: 0370343
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
08
10
2021
received:
18
06
2021
accepted:
10
11
2021
pubmed:
30
1
2022
medline:
4
2
2022
entrez:
29
1
2022
Statut:
ppublish
Résumé
The endometrial microbiota has been linked to several gynecological disorders, including infertility. It has been shown that the microbial profile of endometrium could have a role in fertilization and pregnancy outcomes. In this study we aim to assess the microbial community of endometrial tissue (ET) and endometrial fluid (EF) samples in women receiving in vitro fertilization (IVF) treatment. We also search for possible associations between chronic endometritis (CE) and endometrial microbiota. This was a cohort study involving 25 women aged between 28 and 42 years with both primary and secondary infertility and with at least one IVF failure. The ET and EF sample collection was carried out between September 2016 and November 2018. Each of the participants provided two types of samples-tissue and fluid samples (50 samples in total). A 16S rRNA sequencing was performed on both of the sample types for microbial profile evaluation. CE was diagnosed based on a CD138 immunohistochemistry where CE diagnosis was confirmed in the presence of one or more plasma cells. Microbial profiles of women with and without CE were compared in both sample types separately. We report no differences in the microbial composition and alpha diversity (p Our findings show that genus Lactobacillus dominance is an important factor influencing the microbial composition of ET and fluid samples.
Identifiants
pubmed: 35092013
doi: 10.1111/aogs.14297
pmc: PMC9564573
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
212-220Subventions
Organisme : Ettevõtluse Arendamise Sihtasutus
ID : EU48695
Organisme : Eesti Teadusagentuur
ID : PRG 1076
Organisme : Eesti Teadusagentuur
ID : PRG 687
Organisme : Eesti Teadusagentuur
ID : PUT 1371
Organisme : European Regional Development Fund
ID : Nutika Spetsialiseerumise Stipendium
Organisme : European Regional Development Fund
ID : 15-0012 GENTRANSMED
Organisme : European Molecular Biology Organization
ID : 3573
Organisme : Horizon 2020 Framework Programme
ID : EU952516 (ERIN)
Organisme : Estonian Center of Genomics
ID : 16-0125
Informations de copyright
© 2022 The Authors. Acta Obstetricia et Gynecologica Scandinavica published by John Wiley & Sons Ltd on behalf of Nordic Federation of Societies of Obstetrics and Gynecology (NFOG).
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