Molecular Detection of Avian Influenza Virus in Wild Birds in Morocco, 2016-2019.
Avian influenza
Morocco
Real time RT-PCR
wild birds
Journal
Avian diseases
ISSN: 1938-4351
Titre abrégé: Avian Dis
Pays: United States
ID NLM: 0370617
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
08
08
2021
accepted:
22
10
2021
pubmed:
30
1
2022
medline:
29
6
2022
entrez:
29
1
2022
Statut:
ppublish
Résumé
Avian influenza (AI) is a zoonotic disease significant to both public and animal health, caused by influenza virus A, and affects domestic poultry, wild birds, and mammals including humans. Aquatic birds are considered the natural reservoir of this virus. In 2016, Morocco experienced the first occurrence of low pathogenic H9N2 avian influenza virus (AIV) in poultry; however, no cases were reported in wild birds. The present study aimed to monitor the presence of AIV in wild birds in Morocco in order to trace the possible sources of the viruses affecting poultry. Between 2016 and 2019, 967 samples obtained from 480 birds representing 56 different wild bird species, 20 families, and 8 orders, mostly from Charadriiformes, Anseriformes, Pelecaniformes, and Passeriformes, were collected from various wetlands and relevant ornithologic sites in Morocco. These field samples consisted of 374 cloacal swabs, 321 tracheal swabs, 54 fecal samples, and 218 organ pools including the trachea, lung, liver, spleen, heart, intestine, and brain. The samples were examined for the presence of AIV using TaqMan-based real-time reverse transcriptase-PCR (rRT-PCR) targeting the matrix gene, followed by further subtyping rRT-PCR tests targeting the H1-H16 genes. The AI matrix gene was detected in 18 out of 967 samples (1.86%); positive samples were detected in 17 birds belonging to 10 bird species: two redshanks ( Detección molecular del virus de la influenza aviar en aves silvestres en Marruecos, entre los años 2016 al 2019. La influenza aviar (IA) es una enfermedad zoonótica importante para la salud pública y animal, causada por el virus de la influenza A y afecta a la avicultura comercial, las aves silvestres y los mamíferos, incluyendo a los humanos. Las aves acuáticas se consideran el reservorio natural de este virus. En el año 2016, Marruecos experimentó la primera aparición del virus de la influenza aviar de baja patogenicidad H9N2 en avicultura; sin embargo, no se notificaron casos en aves silvestres. El presente estudio tuvo como objetivo monitorear la presencia del virus de influenza aviar en aves silvestres en Marruecos con el fin de rastrear las posibles fuentes de los virus que afectan a las aves comerciales. Entre los años 2016 y 2019, se recolectaron 967 muestras de 480 aves que representan 56 especies diferentes de aves silvestres, 20 familias y 8 órdenes, principalmente de Charadriiformes, Anseriformes, Pelecaniformes y Passeriformes, de varios humedales y sitios ornitológicos relevantes en Marruecos. Estas muestras de campo consistieron en 374 hisopos cloacales, 321 hisopos traqueales, 54 muestras fecales y 218 conjuntos de órganos que incluyeron tráquea, pulmón, hígado, bazo, corazón, intestino y cerebro. Las muestras se examinaron para detectar la presencia del virus de la influenza aviar mediante transcripción reversa y PCR en tiempo real basada en TaqMan (rRT-PCR) que estivo dirigida al gene de la matriz, seguida de más pruebas de subtipificación de rRT-PCR dirigidas a los genes H1 al H16. El gene de la matriz de influenza aviar se detectó en 18 de 967 muestras (1.86%); Se detectaron muestras positivas en 17 aves pertenecientes a 10 especies de aves: dos archibebes comunes (
Autres résumés
Type: Publisher
(spa)
Detección molecular del virus de la influenza aviar en aves silvestres en Marruecos, entre los años 2016 al 2019. La influenza aviar (IA) es una enfermedad zoonótica importante para la salud pública y animal, causada por el virus de la influenza A y afecta a la avicultura comercial, las aves silvestres y los mamíferos, incluyendo a los humanos. Las aves acuáticas se consideran el reservorio natural de este virus. En el año 2016, Marruecos experimentó la primera aparición del virus de la influenza aviar de baja patogenicidad H9N2 en avicultura; sin embargo, no se notificaron casos en aves silvestres. El presente estudio tuvo como objetivo monitorear la presencia del virus de influenza aviar en aves silvestres en Marruecos con el fin de rastrear las posibles fuentes de los virus que afectan a las aves comerciales. Entre los años 2016 y 2019, se recolectaron 967 muestras de 480 aves que representan 56 especies diferentes de aves silvestres, 20 familias y 8 órdenes, principalmente de Charadriiformes, Anseriformes, Pelecaniformes y Passeriformes, de varios humedales y sitios ornitológicos relevantes en Marruecos. Estas muestras de campo consistieron en 374 hisopos cloacales, 321 hisopos traqueales, 54 muestras fecales y 218 conjuntos de órganos que incluyeron tráquea, pulmón, hígado, bazo, corazón, intestino y cerebro. Las muestras se examinaron para detectar la presencia del virus de la influenza aviar mediante transcripción reversa y PCR en tiempo real basada en TaqMan (rRT-PCR) que estivo dirigida al gene de la matriz, seguida de más pruebas de subtipificación de rRT-PCR dirigidas a los genes H1 al H16. El gene de la matriz de influenza aviar se detectó en 18 de 967 muestras (1.86%); Se detectaron muestras positivas en 17 aves pertenecientes a 10 especies de aves: dos archibebes comunes (
Identifiants
pubmed: 35092236
doi: 10.1637/aviandiseases-D-21-00070
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
29-38Références
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