Ancillary studies on cell blocks from fine needle aspiration specimens of salivary gland lesions: A multi-institutional study.
Milan System for Reporting Cytology
ancillary studies
cell block
fine-needle aspiration
histochemistry stains
immunohistochemistry
in situ hybridization
salivary gland
Journal
Diagnostic cytopathology
ISSN: 1097-0339
Titre abrégé: Diagn Cytopathol
Pays: United States
ID NLM: 8506895
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
revised:
03
01
2022
received:
27
04
2021
accepted:
21
01
2022
pubmed:
30
1
2022
medline:
6
4
2022
entrez:
29
1
2022
Statut:
ppublish
Résumé
Ancillary studies are commonly performed on cell blocks prepared from fine-needle aspiration (FNA) specimens. There are limited studies in application of ancillary studies on cell blocks from salivary gland (SG) FNAs. This multi-institutional study evaluates the role of ancillary studies performed on cell blocks in the diagnosis of SG lesions, and their impact on clinical management. The electronic pathology archives of three large academic institutions were searched for SG FNAs with ancillary studies performed on cell blocks. The patient demographics, FNA site, cytologic diagnosis, ancillary studies, and surgical follow-up were recorded. If needed, the cytologic diagnoses were reclassified as per the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC). 117 SG FNA cases were identified including 3, 10, 11, 6, 23, 4, and 60 cases in MSRSGC categories I, II, III, IVa, IVb, V, VI, respectively with surgical follow-up available ranging from 27% to 100% within each category. Ancillary studies including histochemistry, immunocytochemistry (IHC), and in situ hybridization (ISH) were beneficial in 60%-100% of cases in each category. Risk of malignancy was 100% in both the suspicious for malignancy (V) and malignant (VI) categories. Ancillary studies improved diagnosis in 60% of non-neoplastic cases (II, 6/10), 100% of benign neoplasm cases (IVa, 6/6), and 98.3% of malignant cases (VI, 59/60). Judicious and case-based ancillary studies performed on SG FNA cell blocks with sufficient material can improve the diagnostic yield by further characterization of the atypical/neoplastic cells, particularly in MSRSGC categories IVa-VI.
Sections du résumé
BACKGROUND
BACKGROUND
Ancillary studies are commonly performed on cell blocks prepared from fine-needle aspiration (FNA) specimens. There are limited studies in application of ancillary studies on cell blocks from salivary gland (SG) FNAs. This multi-institutional study evaluates the role of ancillary studies performed on cell blocks in the diagnosis of SG lesions, and their impact on clinical management.
METHOD
METHODS
The electronic pathology archives of three large academic institutions were searched for SG FNAs with ancillary studies performed on cell blocks. The patient demographics, FNA site, cytologic diagnosis, ancillary studies, and surgical follow-up were recorded. If needed, the cytologic diagnoses were reclassified as per the Milan System for Reporting Salivary Gland Cytopathology (MSRSGC).
RESULTS
RESULTS
117 SG FNA cases were identified including 3, 10, 11, 6, 23, 4, and 60 cases in MSRSGC categories I, II, III, IVa, IVb, V, VI, respectively with surgical follow-up available ranging from 27% to 100% within each category. Ancillary studies including histochemistry, immunocytochemistry (IHC), and in situ hybridization (ISH) were beneficial in 60%-100% of cases in each category. Risk of malignancy was 100% in both the suspicious for malignancy (V) and malignant (VI) categories. Ancillary studies improved diagnosis in 60% of non-neoplastic cases (II, 6/10), 100% of benign neoplasm cases (IVa, 6/6), and 98.3% of malignant cases (VI, 59/60).
CONCLUSION
CONCLUSIONS
Judicious and case-based ancillary studies performed on SG FNA cell blocks with sufficient material can improve the diagnostic yield by further characterization of the atypical/neoplastic cells, particularly in MSRSGC categories IVa-VI.
Identifiants
pubmed: 35092649
doi: 10.1002/dc.24939
pmc: PMC9303557
doi:
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
235-252Informations de copyright
© 2022 The Authors. Diagnostic Cytopathology published by Wiley Periodicals LLC.
Références
Am J Clin Pathol. 2015 Jun;143(6):839-53
pubmed: 25972326
Cancer Cytopathol. 2018 Aug;126 Suppl 8:627-642
pubmed: 30156767
Cancer Cytopathol. 2021 Jan;129(1):43-52
pubmed: 32767837
J Am Soc Cytopathol. 2021 Jul-Aug;10(4):414-422
pubmed: 33422456
Diagn Cytopathol. 2017 Sep;45(9):808-819
pubmed: 28411379
Cancer Cytopathol. 2020 Oct;128(10):693-703
pubmed: 32421944
Laryngoscope. 2018 Feb;128(2):398-402
pubmed: 28782105
Cancers (Basel). 2019 Dec 01;11(12):
pubmed: 31805712
Arch Pathol Lab Med. 2015 Dec;139(12):1491-7
pubmed: 26619021
Cancer Cytopathol. 2018 Feb;126(2):101-111
pubmed: 29131559
Diagn Cytopathol. 2022 May;50(5):235-252
pubmed: 35092649
Diagn Cytopathol. 1991;7(3):267-72
pubmed: 1879262
Acta Cytol. 2020;64(1-2):92-102
pubmed: 30909279
Diagn Cytopathol. 2020 Nov;48(11):972-978
pubmed: 32506684
Am J Clin Pathol. 2011 Jul;136(1):45-59
pubmed: 21685031