Gene Co-Expression Network Analysis Identifies Vitamin D-Associated Gene Modules in Adult Normal Rectal Epithelium Following Supplementation.
WGCNA (weighted gene co-expression network analysis)
colorectal cancer
gene correlation network
vitamin D
vitamin D supplementation
Journal
Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621
Informations de publication
Date de publication:
2021
2021
Historique:
received:
27
09
2021
accepted:
02
12
2021
entrez:
31
1
2022
pubmed:
1
2
2022
medline:
1
2
2022
Statut:
epublish
Résumé
Colorectal cancer (CRC) is a common, multifactorial disease. While observational studies have identified an association between lower vitamin D and higher CRC risk, supplementation trials have been inconclusive and the mechanisms by which vitamin D may modulate CRC risk are not well understood. We sought to perform a weighted gene co-expression network analysis (WGCNA) to identify modules present after vitamin D supplementation (when plasma vitamin D level was sufficient) which were absent before supplementation, and then to identify influential genes in those modules. The transcriptome from normal rectal mucosa biopsies of 49 individuals free from CRC were assessed before and after 12 weeks of 3200IU/day vitamin D (Fultium-D3) supplementation using paired-end total RNAseq. While the effects on expression patterns following vitamin D supplementation were subtle,
Identifiants
pubmed: 35096006
doi: 10.3389/fgene.2021.783970
pii: 783970
pmc: PMC8790603
doi:
Types de publication
Journal Article
Langues
eng
Pagination
783970Subventions
Organisme : Cancer Research UK
ID : 12076
Pays : United Kingdom
Organisme : Chief Scientist Office
ID : SCAF/16/01
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M004007/1
Pays : United Kingdom
Organisme : Cancer Research UK
ID : 11378
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00007/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_U127527198
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_U127527198
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K018647/1
Pays : United Kingdom
Informations de copyright
Copyright © 2022 Blackmur, Vaughan-Shaw, Donnelly, Harris, Svinti, Ochocka-Fox, Freile, Walker, Gurran, Reid, Semple, Din, Timofeeva, Dunlop and Farrington.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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