Detection of anti-SARS-CoV-2 antibodies in dried blood spots utilizing manual or automated spot extraction and electrochemiluminescence immunoassay (ECLIA).
COVID‐19
DBS
ECLIA
SARS‐CoV‐2
antibody
automated DBS extraction
Journal
Analytical science advances
ISSN: 2628-5452
Titre abrégé: Anal Sci Adv
Pays: Germany
ID NLM: 9918334986206676
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
05
02
2021
revised:
11
03
2021
accepted:
11
03
2021
pubmed:
1
2
2022
medline:
1
2
2022
entrez:
31
1
2022
Statut:
ppublish
Résumé
Serological test methods to detect anti-SARS-CoV-2 antibodies represent a major measure to manage the pandemic caused by the coronavirus disease 2019 (COVID-19). In this communication, test results obtained from minimal-invasively collected dried blood spot (DBS) specimens, which can be sampled 'at home' without the need of medically trained personnel, are compared to conventionally collected venous blood samples. DBS samples were prepared for analysis either manually or by a card extraction robot, and electrochemiluminescence assay (ECLIA) characteristics, assay readout values as well as stability data covering a period of more than 200 days are provided. Constant anti-SARS-CoV-2 antibody readouts of quality control DBS were obtained over the entire test period using DBS specimens stored under dry and dark conditions. In addition, test results obtained from individuals tested twice within 10 months post-infection indicated a consistent presence of antibodies.
Identifiants
pubmed: 35098125
doi: 10.1002/ansa.202100009
pii: ANSA202100009
pmc: PMC8250974
doi:
Types de publication
Journal Article
Langues
eng
Pagination
440-446Informations de copyright
© 2021 The Authors. Analytical Science Advances published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors have declared no conflict of interest.
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