The VRAC blocker DCPIB directly gates the BK channels and increases intracellular Ca
calcium-activated potassium channel
intracellular calcium
melanoma
pancreatic duct adenocarcinoma
potassium channel openers
volume regulated anion channel
Journal
British journal of pharmacology
ISSN: 1476-5381
Titre abrégé: Br J Pharmacol
Pays: England
ID NLM: 7502536
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
revised:
18
01
2022
received:
30
03
2021
accepted:
24
01
2022
pubmed:
2
2
2022
medline:
10
6
2022
entrez:
1
2
2022
Statut:
ppublish
Résumé
The volume regulated anion channel (VRAC) is known to be involved in different aspects of cancer cell behaviour and response to therapies. For this reason, we investigated the effect of DCPIB, a presumably specific blocker of VRAC, in two types of cancer: pancreatic duct adenocarcinoma (PDAC) and melanoma. We used patch-clamp electrophysiology, supported by Ca DCPIB markedly increased whole-cell currents in Panc-1, MiaPaca2 and IGR39, but not in IGR37 cells. The currents were mostly mediated by K DCPIB directly targeted BK channels and, also, acutely increased intracellular Ca
Sections du résumé
BACKGROUND AND PURPOSE
The volume regulated anion channel (VRAC) is known to be involved in different aspects of cancer cell behaviour and response to therapies. For this reason, we investigated the effect of DCPIB, a presumably specific blocker of VRAC, in two types of cancer: pancreatic duct adenocarcinoma (PDAC) and melanoma.
EXPERIMENTAL APPROACH
We used patch-clamp electrophysiology, supported by Ca
KEY RESULTS
DCPIB markedly increased whole-cell currents in Panc-1, MiaPaca2 and IGR39, but not in IGR37 cells. The currents were mostly mediated by K
CONCLUSION AND IMPLICATIONS
DCPIB directly targeted BK channels and, also, acutely increased intracellular Ca
Substances chimiques
Anions
0
Large-Conductance Calcium-Activated Potassium Channels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3452-3469Informations de copyright
© 2022 The British Pharmacological Society.
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