Analysis of RNA by Northern Blotting.

Blotting, Northern Nucleic Acid Hybridization / methods RNA / analysis Sepharose

Journal

Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530

Informations de publication

Date de publication:
01 02 2022
Historique:
entrez: 2 2 2022
pubmed: 3 2 2022
medline: 23 4 2022
Statut: epublish

Résumé

Northern hybridization is used to measure the amount and size of RNAs transcribed from eukaryotic genes and to estimate their abundance. No other method is capable of obtaining these pieces of information simultaneously from a large number of RNA preparations; northern analysis is therefore fundamental to studies of gene expression in eukaryotic cells. To prepare a northern blot for hybridization, RNA must be separated according to size through a denaturing agarose or polyacrylamide gel and transferred to a solid support in a way that preserves its topological distribution within the gel. These important steps in northern analysis are discussed here.

Identifiants

DOI: 10.1101/pdb.top101741 PMID: 35105788
pubmed: 35105788
pii: 2022/2/pdb.top101741
doi: 10.1101/pdb.top101741
doi:

Substances chimiques

RNA 63231-63-0
Sepharose 9012-36-6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2022 Cold Spring Harbor Laboratory Press.

Auteurs

Michael R Green (MR)

Joseph Sambrook (J)

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Technique de Northern › Analysis of RNA by Northern Blotting.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Hybridation d'acides nucléiques › Analysis of RNA by Northern Blotting.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Analysis of RNA by Northern Blotting.
questionsmedicales.fr › Glucides › Polyosides › Agarose › Analysis of RNA by Northern Blotting.