On the role of phase separation in the biogenesis of membraneless compartments.
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
01 03 2022
01 03 2022
Historique:
revised:
07
01
2022
received:
14
10
2021
accepted:
10
01
2022
pubmed:
3
2
2022
medline:
9
3
2022
entrez:
2
2
2022
Statut:
ppublish
Résumé
Molecular mechanistic biology has ushered us into the world of life's building blocks, revealing their interactions in macromolecular complexes and inspiring strategies for detailed functional interrogations. The biogenesis of membraneless cellular compartments, functional mesoscale subcellular locales devoid of strong internal order and delimiting membranes, is among mechanistic biology's most demanding current challenges. A developing paradigm, biomolecular phase separation, emphasizes solvation of the building blocks through low-affinity, weakly adhesive unspecific interactions as the driver of biogenesis of membraneless compartments. Here, I discuss the molecular underpinnings of the phase separation paradigm and demonstrate that validating its assumptions is much more challenging than hitherto appreciated. I also discuss that highly specific interactions, rather than unspecific ones, appear to be the main driver of biogenesis of subcellular compartments, while phase separation may be harnessed locally in selected instances to generate material properties tailored for specific functions, as exemplified by nucleocytoplasmic transport.
Identifiants
pubmed: 35107832
doi: 10.15252/embj.2021109952
pmc: PMC8886532
doi:
Substances chimiques
Macromolecular Substances
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e109952Informations de copyright
© 2022 The Author. Published under the terms of the CC BY 4.0 license.
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