Microbiome changes in the gastric mucosa and gastric juice in different histological stages of
16s RNA gene sequencing
Gastric juice
Gastric mucosa
Histological stages
Microbiota
Stomach neoplasms
Journal
World journal of gastroenterology
ISSN: 2219-2840
Titre abrégé: World J Gastroenterol
Pays: United States
ID NLM: 100883448
Informations de publication
Date de publication:
21 Jan 2022
21 Jan 2022
Historique:
received:
26
11
2021
revised:
14
12
2021
accepted:
11
01
2022
entrez:
3
2
2022
pubmed:
4
2
2022
medline:
5
2
2022
Statut:
ppublish
Résumé
The gastric microbiota in patients with gastric cancer (GC) has received increasing attention, but the profiling of the gastric microbiome through the histological stages of gastric tumorigenesis remains poorly understood, especially for patients with To characterize microbial profiles of gastric mucosa and juice for HPNGC carcinogenesis and identify distinct taxa in precancerous lesions. The 16S rRNA gene analysis was performed on gastric mucosa from 134 The diversity and composition of the bacterial microbiota in the gastric mucosa changed progressively across stages of gastric carcinogenesis. The diversity of the gastric mucosa microbiota was found to be significantly lower in the IM and Dys groups than in the SG group, and the patients with GC had the lowest bacterial community richness ( Our results provide insights into potential taxonomic biomarkers for HPNGC and its precancerous stages and assist in predicting the prognosis of IM and Dys based on the mucosal microbiota profile.
Sections du résumé
BACKGROUND
BACKGROUND
The gastric microbiota in patients with gastric cancer (GC) has received increasing attention, but the profiling of the gastric microbiome through the histological stages of gastric tumorigenesis remains poorly understood, especially for patients with
AIM
OBJECTIVE
To characterize microbial profiles of gastric mucosa and juice for HPNGC carcinogenesis and identify distinct taxa in precancerous lesions.
METHODS
METHODS
The 16S rRNA gene analysis was performed on gastric mucosa from 134
RESULTS
RESULTS
The diversity and composition of the bacterial microbiota in the gastric mucosa changed progressively across stages of gastric carcinogenesis. The diversity of the gastric mucosa microbiota was found to be significantly lower in the IM and Dys groups than in the SG group, and the patients with GC had the lowest bacterial community richness (
CONCLUSION
CONCLUSIONS
Our results provide insights into potential taxonomic biomarkers for HPNGC and its precancerous stages and assist in predicting the prognosis of IM and Dys based on the mucosal microbiota profile.
Identifiants
pubmed: 35110955
doi: 10.3748/wjg.v28.i3.365
pmc: PMC8771614
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
365-380Informations de copyright
©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict-of-interest statement: No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article.
Références
BMC Microbiol. 2019 Dec 9;19(1):275
pubmed: 31815623
Helicobacter. 2019 Feb;24(1):e12547
pubmed: 30440093
Lancet Oncol. 2006 Mar;7(3):211-22
pubmed: 16510330
Helicobacter. 2011 Oct;16(5):382-8
pubmed: 21923684
Curr Protoc Bioinformatics. 2011 Dec;Chapter 10:Unit 10.7.
pubmed: 22161565
Gut. 2002 Jul;51(1):130-1
pubmed: 12077106
Nature. 2010 Mar 4;464(7285):59-65
pubmed: 20203603
BMC Cancer. 2010 Jul 17;10:374
pubmed: 20637122
Cancer Causes Control. 1999 Feb;10(1):77-83
pubmed: 10334646
Rev Bras Ter Intensiva. 2016 Jun;28(2):195-8
pubmed: 27410414
Lancet Oncol. 2012 Jun;13(6):607-15
pubmed: 22575588
Dig Dis Sci. 2019 May;64(5):1193-1203
pubmed: 30535886
Aliment Pharmacol Ther. 2020 Apr;51(8):770-780
pubmed: 32133670
Genome Biol Evol. 2014 Mar;6(3):703-13
pubmed: 24625961
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Gut Microbes. 2020 Sep 2;11(5):1220-1230
pubmed: 32449430
Eur J Gastroenterol Hepatol. 2016 Mar;28(3):261-6
pubmed: 26657453
Am J Surg Pathol. 1996 Oct;20(10):1161-81
pubmed: 8827022
EBioMedicine. 2019 Feb;40:336-348
pubmed: 30584008
Clin Gastroenterol Hepatol. 2006 Apr;4(4):416-25
pubmed: 16616344
Cancer Res. 1993 Mar 15;53(6):1317-21
pubmed: 8443811
Cancer Lett. 1997 Mar 19;114(1-2):259-61
pubmed: 9103306
Nutrients. 2014 Dec 24;7(1):17-44
pubmed: 25545101
Sci Rep. 2016 Feb 10;6:20701
pubmed: 26860194
J Cancer Prev. 2015 Mar;20(1):25-40
pubmed: 25853101
Gut. 2020 Sep;69(9):1598-1607
pubmed: 31857433
Cancer. 2009 May 15;115(10):2071-80
pubmed: 19280589
Hepatogastroenterology. 1998 Nov-Dec;45(24):2432-6
pubmed: 9951939
Cancer Sci. 2003 Jun;94(6):492-6
pubmed: 12824872
Carcinogenesis. 2000 Apr;21(4):753-6
pubmed: 10753212
Front Microbiol. 2020 May 29;11:997
pubmed: 32547510
Proc Natl Acad Sci U S A. 2006 Jan 17;103(3):732-7
pubmed: 16407106
J Med Microbiol. 2009 Apr;58(Pt 4):509-516
pubmed: 19273648
Gut. 2018 Jun;67(6):1024-1032
pubmed: 28765474
Helicobacter. 2014 Dec;19(6):407-16
pubmed: 25052961
Int J Oncol. 2013 Jan;42(1):5-18
pubmed: 23165522
Front Cell Infect Microbiol. 2017 Jul 06;7:302
pubmed: 28730144
Front Cell Infect Microbiol. 2018 Dec 12;8:433
pubmed: 30619779
Dig Dis Sci. 2021 May;66(5):1673-1682
pubmed: 32591968
World J Gastroenterol. 2009 May 14;15(18):2204-13
pubmed: 19437559
Carcinogenesis. 2004 Aug;25(8):1477-84
pubmed: 14742316
Front Microbiol. 2020 Jul 07;11:1548
pubmed: 32733423
World J Gastroenterol. 2006 Jan 21;12(3):354-62
pubmed: 16489633
Int J Cancer. 2015 Jan 15;136(2):487-90
pubmed: 24889903
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
Sci Rep. 2018 Feb 27;8(1):3685
pubmed: 29487347
Sci Rep. 2017 Nov 21;7(1):15957
pubmed: 29162924
Gastric Cancer. 2010 Mar;13(1):1-10
pubmed: 20373070