A field-friendly alternative to freeze-drying faeces for glucocorticoid metabolite analyses of African wild dogs (

Lyophilisation Non-invasive Steroids Stress Wild dog faeces

Journal

MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829

Informations de publication

Date de publication:
2022
Historique:
received: 11 02 2021
accepted: 14 01 2022
entrez: 3 2 2022
pubmed: 4 2 2022
medline: 4 2 2022
Statut: epublish

Résumé

Endocrine studies using faeces as hormone matrix have become increasingly popular to examine adrenocortical activity in wildlife. A prerequisite for this approach is to minimize alteration of faecal glucocorticoid metabolite (fGCM) composition post-defecation. This is done by freezing the collected material as soon as possible after collection, and removing moisture from the frozen faecal samples afterwards (usually by freeze-drying). In remote areas, freeze-drying opportunities are often limited, and in the case of the African wild dog (

Identifiants

pubmed: 35111576
doi: 10.1016/j.mex.2022.101623
pii: S2215-0161(22)00008-5
pmc: PMC8790624
doi:

Types de publication

Journal Article

Langues

eng

Pagination

101623

Informations de copyright

© 2022 The Authors. Published by Elsevier B.V.

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Auteurs

Gabriella Postiglione (G)

Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Italy.

Pier Attilio Accorsi (PA)

Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Italy.

Andre Ganswindt (A)

Mammal Research Institute (MRI), Department of Zoology and Entomology, Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa.
Endocrine Research Laboratory, Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa.
Centre for Veterinary Wildlife Studies, Faculty of Veterinary Science, University of Pretoria, South Africa.

Bruce Crossey (B)

Mammal Research Institute (MRI), Department of Zoology and Entomology, Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa.
Endocrine Research Laboratory, Faculty of Natural and Agricultural Sciences, University of Pretoria, South Africa.

Classifications MeSH