Sperm morphology and DNA fragmentation after zona pellucida selection.
sperm DNA fragmentation (SDF)
sperm morphology
sperm selection
zona adhesion
zona pellucida
Journal
Reproduction & fertility
ISSN: 2633-8386
Titre abrégé: Reprod Fertil
Pays: England
ID NLM: 101778727
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
06
07
2021
accepted:
12
08
2021
entrez:
4
2
2022
pubmed:
5
2
2022
medline:
5
2
2022
Statut:
epublish
Résumé
Sperm DNA fragmentation (SDF) and sperm morphological defects can negatively affect ART outcomes. Consequently, there is a need for additional semen processing technique that accounts for sperm DNA status and morphology prior to ICSI. The objective was to evaluate the efficacy of an additional zona pellucida adhesion-based sperm selection for obtaining sperm populations with a high percentage of normal morphology and DNA integrity as compared to native semen and routine swim-up preparation. Semen samples from 78 normozoospermic men were subjected to swim up and placed in petri dishes coated with 48 acid-solubilized zonae pellucidae. Sperm DNA fragmentation and morphology were assessed in the native semen, the swim-up samples, and the zona-adhered spermatozoa from each patient. The mean sperm DNA fragmentation of the zona-selected spermatozoa (3.5 ± 0.7%) was significantly lower than the swim-up samples (15.3 ± 5.2%) ( High level of DNA folding known as sperm DNA fragmentation (SDF) inside each sperm and defects in the shape, size, and structure of the sperm can negatively affect assisted reproduction treatment (ART) outcomes. Consequently, there is a need for additional semen processing techniques that account for sperm quality prior to ART. Our team designed a simple technique using proteins from the coat around the egg (zona pellucida) to enhance sperm selection procedures based on natural sperm-egg interactions. Using this technique in combination with the most common techniques used in ART yields a significantly higher percentage of sperm with normal shape, size, and structure and a decreased level of DNA fragmentation. This sperm zona-selection technique would be beneficial if introduced in the ART practice to yield sperm with higher fertilization potential.
Identifiants
pubmed: 35118392
doi: 10.1530/RAF-21-0041
pii: RAF-21-0041
pmc: PMC8801029
doi:
Types de publication
Journal Article
Langues
eng
Pagination
221-230Informations de copyright
© The authors.
Références
J Reprod Fertil. 1992 Jan;94(1):71-84
pubmed: 1552494
Clin Exp Reprod Med. 2017 Sep;44(3):132-140
pubmed: 29026719
Fertil Steril. 2011 Feb;95(2):815-8
pubmed: 20971463
Arch Gynecol Obstet. 2012 Nov;286(5):1315-22
pubmed: 22760592
J In Vitro Fert Embryo Transf. 1988 Jun;5(3):134-43
pubmed: 3171321
Fertil Steril. 2014 Dec;102(6):1502-7
pubmed: 25458617
Hum Reprod. 1999 Mar;14(3):726-30
pubmed: 10221704
Transl Androl Urol. 2020 Apr;9(2):405-415
pubmed: 32420146
Fertil Steril. 2015 Apr;103(4):910-6
pubmed: 25747135
Fertil Steril. 2001 Apr;75(4):674-7
pubmed: 11287017
J Assist Reprod Genet. 2006 Sep-Oct;23(9-10):367-76
pubmed: 17019633
Fertil Steril. 2008 Sep;90(3):889-90
pubmed: 17481620
J Assist Reprod Genet. 2012 Jun;29(6):557-63
pubmed: 22411295
J Androl. 2010 Nov-Dec;31(6):566-72
pubmed: 20133967
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Biol Reprod. 2008 Apr;78(4):761-72
pubmed: 18199884
Fertil Steril. 2002 Aug;78(2):313-8
pubmed: 12137868
Fertil Steril. 1988 Jan;49(1):112-7
pubmed: 3335257
Fertil Steril. 2013 Jul;100(1):75-80
pubmed: 23562046
Mol Hum Reprod. 2012 Jan;18(1):14-21
pubmed: 21911476
Hum Reprod. 2007 Jun;22(6):1597-602
pubmed: 17369294
Reprod Biomed Online. 2019 Jun;38(6):951-960
pubmed: 30979611
Fertil Steril. 1991 Dec;56(6):1156-61
pubmed: 1743337
Asian J Androl. 2018 Jan-Feb;20(1):75-79
pubmed: 28675153
Fertil Steril. 1988 Apr;49(4):688-97
pubmed: 3350165
J Assist Reprod Genet. 2015 Jan;32(1):17-26
pubmed: 25392073
J Assist Reprod Genet. 1999 Sep;16(8):436-42
pubmed: 10478324
Mol Reprod Dev. 1991 Dec;30(4):346-52
pubmed: 1751039
Andrologia. 2015 Jun;47(5):519-24
pubmed: 24890860
Hum Reprod. 1994 Apr;9(4):673-9
pubmed: 8046022
Fertil Steril. 2003 Dec;80(6):1413-9
pubmed: 14667877
Fertil Steril. 2014 Oct;102(4):998-1005.e8
pubmed: 25190048
J Assist Reprod Genet. 2019 Oct;36(10):2057-2066
pubmed: 31418108
Hum Reprod. 2003 Apr;18(4):802-7
pubmed: 12660274
Andrologia. 1990 Jan-Feb;22(1):12-20
pubmed: 2281873
Reprod Biomed Online. 2015 Feb;30(2):120-7
pubmed: 25530036
Andrology. 2017 Sep;5(5):845-862
pubmed: 28692759
PLoS One. 2016 Nov 10;11(11):e0165125
pubmed: 27832085
Reprod Biomed Online. 2009 Dec;19(6):802-7
pubmed: 20031020
Int J Androl. 1995 Jun;18 Suppl 1:58-61
pubmed: 7558390
Fertil Steril. 2010 Feb;93(2):672-4
pubmed: 19878934
Andrologia. 2012 Feb;44(1):59-65
pubmed: 21592172
Hum Reprod. 2018 Aug 1;33(8):1388-1393
pubmed: 30007319
Fertil Steril. 1996 Mar;65(3):637-44
pubmed: 8774300
Fertil Steril. 2014 Mar;101(3):647-52
pubmed: 24360607
Fertil Steril. 2003 Jun;79 Suppl 3:1616-24
pubmed: 12801568
Hum Reprod. 1989 Aug;4(6):696-701
pubmed: 2778055
Hum Reprod. 1999 Apr;14(4):1039-49
pubmed: 10221239
Asian J Androl. 2011 Mar;13(2):197-8
pubmed: 21240292
Fertil Steril. 2006 Jun;85(6):1697-707
pubmed: 16682031
Fertil Steril. 1988 Nov;50(5):782-8
pubmed: 3141221
Mol Cell Biol. 2007 Oct;27(19):6794-805
pubmed: 17664285
Fertil Steril. 1997 Sep;68(3):519-24
pubmed: 9314926
J Reprod Infertil. 2015 Jan-Mar;16(1):1-2
pubmed: 25717428
Fertil Steril. 2019 Apr;111(4):672-673
pubmed: 30929725
Int Braz J Urol. 2012 Sep-Oct;38(5):576-94
pubmed: 23131516
Sci Rep. 2019 May 16;9(1):7492
pubmed: 31097741
J Assist Reprod Genet. 2013 Dec;30(12):1611-5
pubmed: 24141830
Fertil Steril. 1995 Jun;63(6):1329-32
pubmed: 7750609
J Assist Reprod Genet. 2016 May;33(5):597-601
pubmed: 26941098
Fertil Steril. 2009 Feb;91(2):632-8
pubmed: 18206147
Fertil Steril. 2010 Sep;94(4):1465-1472
pubmed: 19540481
Fertil Steril. 1997 Feb;67(2):362-71
pubmed: 9022616
J Assist Reprod Genet. 2014 Dec;31(12):1655-63
pubmed: 25227693
Hum Reprod. 2000 Mar;15(3):702-8
pubmed: 10686223
J Ovarian Res. 2020 May 2;13(1):52
pubmed: 32359369
Adv Exp Med Biol. 2014;791:151-72
pubmed: 23955678
Hum Reprod. 2012 Oct;27(10):2908-17
pubmed: 22791753
Turk J Urol. 2017 Dec;43(4):393-400
pubmed: 29201498
Fertil Steril. 1990 Sep;54(3):497-503
pubmed: 2397792