Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle-Based Approach for Microscale Cell Culture.
homeostasis
microscale cell culture
oxygen microenvironment
physioxia
supply-demand balance
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
revised:
20
11
2021
received:
11
10
2021
pubmed:
5
2
2022
medline:
8
4
2022
entrez:
4
2
2022
Statut:
ppublish
Résumé
Oxygen levels in vivo are autonomously regulated by a supply-demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply-demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi-liquid-phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands-primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut).
Identifiants
pubmed: 35118834
doi: 10.1002/advs.202104510
pmc: PMC8981459
doi:
Substances chimiques
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2104510Subventions
Organisme : NCI NIH HHS
ID : R01 CA181648
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA014520
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA186134
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB010039
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI154940
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM124774
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA247479
Pays : United States
Organisme : NIH HHS
ID : S10 OD023526
Pays : United States
Organisme : NHGRI NIH HHS
ID : T32 HG002760
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM140935
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA185251
Pays : United States
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Angew Chem Int Ed Engl. 2010 Aug 9;49(34):5846-68
pubmed: 20572214
Lab Chip. 2014 Nov 21;14(22):4305-18
pubmed: 25251498
Trends Biotechnol. 2014 Nov;32(11):556-563
pubmed: 25282035
Nat Commun. 2019 Mar 6;10(1):1095
pubmed: 30842556
J Stem Cells. 2015;10(2):97-106
pubmed: 27125138
Sci Adv. 2020 Apr 17;6(16):eaay9919
pubmed: 32494607
Toxins (Basel). 2018 Jul 02;10(7):
pubmed: 30004412
ISME J. 2013 Jul;7(7):1256-61
pubmed: 23677008
Trends Immunol. 2019 Feb;40(2):174
pubmed: 30609968
Ann N Y Acad Sci. 1968 Jan;146(1):119-37
pubmed: 5238627
Lab Chip. 2017 Apr 11;17(8):1436-1441
pubmed: 28322402
Structure. 2008 Jul;16(7):1105-15
pubmed: 18611383
Anal Chem. 2009 Apr 15;81(8):3008-16
pubmed: 19284775
Pflugers Arch. 2016 Jan;468(1):77-84
pubmed: 26206140
Biomed Microdevices. 2016 Dec;18(6):114
pubmed: 27921279
Am J Physiol Cell Physiol. 2008 Jul;295(1):C180-91
pubmed: 18480296
ACS Appl Mater Interfaces. 2018 May 23;10(20):17065-17070
pubmed: 29738227
Biomedicines. 2018 May 15;6(2):
pubmed: 29762526
Curr Opin Biotechnol. 2014 Feb;25:95-102
pubmed: 24484886
Adv Microb Physiol. 2002;46:111-53
pubmed: 12073652
Birth Defects Res C Embryo Today. 2003 Feb;69(1):73-82
pubmed: 12768659
Nature. 2004 Jan 29;427(6973):441-4
pubmed: 14749831
Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):E5926-E5933
pubmed: 29895687
Lab Chip. 2012 Apr 7;12(7):1224-37
pubmed: 22318426
Science. 2009 May 22;324(5930):1029-33
pubmed: 19460998
Sci Adv. 2019 Jun 05;5(6):eaav8002
pubmed: 31183401
Nature. 2014 Feb 27;506(7489):498-502
pubmed: 24463512
Cell Mol Gastroenterol Hepatol. 2018 Apr 24;5(4):659-668
pubmed: 29713674
Free Radic Biol Med. 2011 Aug 1;51(3):700-12
pubmed: 21664270
Sci Rep. 2019 Sep 24;9(1):13748
pubmed: 31551501
Cell. 2017 Apr 20;169(3):538-546.e12
pubmed: 28431251
Mol Microbiol. 2008 Jan;67(1):129-42
pubmed: 18047569
J Cell Mol Med. 2011 Jun;15(6):1239-53
pubmed: 21251211
Fungal Genet Biol. 2017 Dec;109:1-6
pubmed: 28989089
Expert Opin Drug Metab Toxicol. 2008 Apr;4(4):395-411
pubmed: 18433344
J Clin Invest. 2016 Oct 3;126(10):3716-3724
pubmed: 27454299
Biochim Biophys Acta. 2016 Aug;1866(1):76-86
pubmed: 27343712
Nat Biotechnol. 2014 Aug;32(8):760-72
pubmed: 25093883
Am J Pathol. 2004 Jun;164(6):1875-82
pubmed: 15161623
J R Soc Interface. 2014 Jul 6;11(96):
pubmed: 24829281
J Vis Exp. 2015 Mar 06;(97):
pubmed: 25866936
Nat Commun. 2017 Oct 10;8(1):816
pubmed: 29018186
Lab Chip. 2010 Dec 7;10(23):3291-5
pubmed: 20938500
Nat Commun. 2016 May 11;7:11535
pubmed: 27168102
Biomed Microdevices. 2012 Feb;14(1):145-52
pubmed: 21947550
Mol Cell Pediatr. 2016 Dec;3(1):14
pubmed: 27002817
Cell Host Microbe. 2008 Nov 13;4(5):447-57
pubmed: 18996345
N Engl J Med. 2011 Feb 17;364(7):656-65
pubmed: 21323543
Am J Physiol Cell Physiol. 2015 Nov 1;309(9):C569-79
pubmed: 26310815
Oncogenesis. 2018 Jan 24;7(1):10
pubmed: 29362402
J Clin Invest. 1978 Feb;61(2):480-8
pubmed: 202614
Lab Chip. 2018 Sep 11;18(18):2710-2719
pubmed: 30069559
Lab Chip. 2004 Apr;4(2):91-7
pubmed: 15052346
Chem Soc Rev. 2010 Mar;39(3):1036-48
pubmed: 20179823
J Bacteriol. 2000 Jun;182(12):3559-71
pubmed: 10852890
Gut. 2015 Jun;64(6):911-20
pubmed: 25007816
SLAS Technol. 2019 Dec;24(6):535-542
pubmed: 31180792
Clin Microbiol Rev. 2007 Oct;20(4):593-621
pubmed: 17934076
Free Radic Biol Med. 2017 Dec;113:311-322
pubmed: 29032224
Br J Radiol. 2014 Mar;87(1035):20130676
pubmed: 24588669
Adv Sci (Weinh). 2022 Apr;9(10):e2104510
pubmed: 35118834