The effect of hypoxia on myogenic differentiation and multipotency of the skeletal muscle-derived stem cells in mice.
Differentiation
Hypoxia
Multipotency
Muscle Stem cells
Proliferation
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
05 02 2022
05 02 2022
Historique:
received:
01
11
2021
accepted:
20
01
2022
entrez:
6
2
2022
pubmed:
7
2
2022
medline:
25
3
2022
Statut:
epublish
Résumé
Skeletal muscle-derived stem cells (SC) have become a promising approach for investigating myogenic differentiation and optimizing tissue regeneration. Muscle regeneration is performed by SC, a self-renewal cell population underlying the basal lamina of muscle fibers. Here, we examined the impact of hypoxia condition on the regenerative capacity of SC either in their native microenvironment or via isolation in a monolayer culture using ectopic differentiation inductions. Furthermore, the effect of low oxygen tension on myogenic differentiation protocols of the myoblasts cell line C2C12 was examined. Hind limb muscles of wild type mice were processed for both SC/fiber isolation and myoblast extraction using magnetic beads. SC were induced for myogenic, adipogenic and osteogenic commitments under normoxic (21% O The data revealed enhanced SC proliferation and motility following differentiation induction after 48 h under hypoxia. Following myogenic induction, the number of undifferentiated cells positive for Pax7 were increased at 72 h under hypoxia. Hypoxia upregulated MyoD and downregulated Myogenin expression at day-7 post-myogenic induction. Hypoxia promoted both SC adipogenesis and osteogenesis under respective induction as shown by using Oil Red O and Alizarin Red S staining. The expression of adipogenic markers; peroxisome proliferator activated receptor gamma (PPARγ) and fatty acid-binding protein 4 (FABP4) were upregulated under hypoxia up to day 14 compared to normoxic condition. Enhanced osteogenic differentiation was detected under hypoxic condition via upregulation of osteocalcin and osteopontin expression up to day 14 as well as, increased calcium deposition at day 21. Hypoxia exposure increases the number of adipocytes and the size of fat vacuoles per adipocyte compared to normoxic culture. Combining the differentiation medium with dexamethasone under hypoxia improves the efficiency of the myogenic differentiation protocol of C2C12 by increasing the length of the myotubes. Hypoxia exposure increases cell resources for clinical applications and promotes SC multipotency and thus beneficial for tissue regeneration.
Sections du résumé
BACKGROUND
Skeletal muscle-derived stem cells (SC) have become a promising approach for investigating myogenic differentiation and optimizing tissue regeneration. Muscle regeneration is performed by SC, a self-renewal cell population underlying the basal lamina of muscle fibers. Here, we examined the impact of hypoxia condition on the regenerative capacity of SC either in their native microenvironment or via isolation in a monolayer culture using ectopic differentiation inductions. Furthermore, the effect of low oxygen tension on myogenic differentiation protocols of the myoblasts cell line C2C12 was examined.
METHODS
Hind limb muscles of wild type mice were processed for both SC/fiber isolation and myoblast extraction using magnetic beads. SC were induced for myogenic, adipogenic and osteogenic commitments under normoxic (21% O
RESULTS
The data revealed enhanced SC proliferation and motility following differentiation induction after 48 h under hypoxia. Following myogenic induction, the number of undifferentiated cells positive for Pax7 were increased at 72 h under hypoxia. Hypoxia upregulated MyoD and downregulated Myogenin expression at day-7 post-myogenic induction. Hypoxia promoted both SC adipogenesis and osteogenesis under respective induction as shown by using Oil Red O and Alizarin Red S staining. The expression of adipogenic markers; peroxisome proliferator activated receptor gamma (PPARγ) and fatty acid-binding protein 4 (FABP4) were upregulated under hypoxia up to day 14 compared to normoxic condition. Enhanced osteogenic differentiation was detected under hypoxic condition via upregulation of osteocalcin and osteopontin expression up to day 14 as well as, increased calcium deposition at day 21. Hypoxia exposure increases the number of adipocytes and the size of fat vacuoles per adipocyte compared to normoxic culture. Combining the differentiation medium with dexamethasone under hypoxia improves the efficiency of the myogenic differentiation protocol of C2C12 by increasing the length of the myotubes.
CONCLUSIONS
Hypoxia exposure increases cell resources for clinical applications and promotes SC multipotency and thus beneficial for tissue regeneration.
Identifiants
pubmed: 35123554
doi: 10.1186/s13287-022-02730-5
pii: 10.1186/s13287-022-02730-5
pmc: PMC8817503
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
56Informations de copyright
© 2022. The Author(s).
Références
Wound Repair Regen. 2013 May-Jun;21(3):382-94
pubmed: 23627495
J Vis Exp. 2013 Mar 22;(73):e50074
pubmed: 23542587
Methods Mol Biol. 2013;946:431-68
pubmed: 23179849
Expert Rev Mol Med. 2009 Jun 25;11:e18
pubmed: 19555515
C R Biol. 2007 Jun-Jul;330(6-7):530-3
pubmed: 17631448
Annu Rev Pathol. 2013 Jan 24;8:441-75
pubmed: 23121053
J Anat. 2021 Aug;239(2):336-350
pubmed: 33641201
J Cell Sci. 2008 Sep 15;121(Pt 18):2975-82
pubmed: 18768931
Diabetes. 2007 Apr;56(4):901-11
pubmed: 17395738
Physiol Rev. 2013 Jan;93(1):23-67
pubmed: 23303905
Mol Cell Biol. 2012 Jan;32(1):36-49
pubmed: 22006022
Stem Cells Dev. 2007 Oct;16(5):857-68
pubmed: 17999606
Cell Death Dis. 2019 Jul 18;10(8):552
pubmed: 31320610
Mol Cell Biol. 2012 Jun;32(12):2300-11
pubmed: 22493066
Hum Gene Ther. 2018 Oct;29(10):1098-1105
pubmed: 30132372
Med Hypotheses. 2007;69(3):629-36
pubmed: 17395396
Cell Stem Cell. 2008 Jan 10;2(1):22-31
pubmed: 18371418
Dev Cell. 2002 Mar;2(3):331-41
pubmed: 11879638
J Stem Cell Res Ther. 2012 Nov;Suppl 11:
pubmed: 24524010
Mol Cell Biol. 2005 Apr;25(8):3040-55
pubmed: 15798192
Scientifica (Cairo). 2016;2016:4516920
pubmed: 27379197
Curr Opin Cell Biol. 2007 Dec;19(6):628-33
pubmed: 17996437
J Cell Sci. 2000 Jun;113 ( Pt 12):2299-308
pubmed: 10825301
Am J Physiol. 1989 Apr;256(4 Pt 1):C701-11
pubmed: 2650560
J Clin Invest. 2018 Jun 1;128(6):2339-2355
pubmed: 29533927
PLoS One. 2012;7(11):e49860
pubmed: 23166781
Mol Metab. 2016 Sep 28;5(12):1149-1161
pubmed: 27900258
Cytotechnology. 2009 Dec;61(3):93-107
pubmed: 20091346
Pak J Biol Sci. 2017;20(1):1-11
pubmed: 29023009
J Biophys Biochem Cytol. 1961 Feb;9:493-5
pubmed: 13768451
Curr Opin Clin Nutr Metab Care. 2010 May;13(3):243-8
pubmed: 20098319
Nature. 1977 Dec 22-29;270(5639):725-7
pubmed: 563524
Cell Biol Int. 2008 Aug;32(8):871-8
pubmed: 18468460
Cell Mol Life Sci. 2001 Jul;58(8):1150-8
pubmed: 11529507
Stem Cell Res Ther. 2011 Feb 23;2(1):9
pubmed: 21371354
Development. 2012 Aug;139(16):2857-65
pubmed: 22764051
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9306-11
pubmed: 11459935
Acta Histochem. 2017 Oct;119(8):786-794
pubmed: 29037777
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
MMWR Morb Mortal Wkly Rep. 2009 Oct 16;58(40):1119-22
pubmed: 19834452
Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5857-62
pubmed: 20231451
J Biol Chem. 2002 Dec 20;277(51):49831-40
pubmed: 12244043
Bio Protoc. 2016 Nov 5;6(21):
pubmed: 28573164
PLoS One. 2016 Jul 21;11(7):e0158860
pubmed: 27442119
Genes Dev. 2001 Nov 1;15(21):2865-76
pubmed: 11691837
J Biol Chem. 2004 Apr 16;279(16):16332-8
pubmed: 14754880
J Cell Mol Med. 2011 Jun;15(6):1239-53
pubmed: 21251211
Cell Prolif. 2008 Apr;41(2):193-207
pubmed: 18336467
Exp Cell Res. 2021 Feb 1;399(1):112434
pubmed: 33340494
World J Stem Cells. 2020 Dec 26;12(12):1667-1690
pubmed: 33505607
Am J Stem Cells. 2012 Nov 30;1(3):253-63
pubmed: 23671812
Science. 1985 Nov 15;230(4727):758-66
pubmed: 2414846
Cell Death Discov. 2021 Feb 17;7(1):35
pubmed: 33597503
Sci Rep. 2020 Mar 24;10(1):5363
pubmed: 32210313
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15603-7
pubmed: 9861016
J Neurosci. 2000 Oct 1;20(19):7370-6
pubmed: 11007895
Cold Spring Harb Symp Quant Biol. 2011;76:101-11
pubmed: 21960527
Curr Opin Neurol. 2011 Oct;24(5):415-22
pubmed: 21892079
Stem Cells Int. 2012;2012:282348
pubmed: 22220178
Int J Med Sci. 2017 Apr 8;14(5):434-443
pubmed: 28539819
Mol Cell Endocrinol. 2011 Jan 30;332(1-2):38-47
pubmed: 20884321
J Allergy Clin Immunol. 2010 Feb;125(2 Suppl 2):S336-44
pubmed: 20061008
Tissue Eng Part A. 2011 Jul;17(13-14):1747-58
pubmed: 21438665
J Neurosci. 2000 Oct 1;20(19):7377-83
pubmed: 11007896
Bioact Mater. 2021 Mar 21;6(10):3485-3495
pubmed: 33817422
Tissue Eng Part B Rev. 2010 Apr;16(2):159-68
pubmed: 19698058
PLoS One. 2012;7(9):e46483
pubmed: 23029528
Skelet Muscle. 2013 Jan 31;3(1):2
pubmed: 23369649
Differentiation. 2001 Oct;68(4-5):245-53
pubmed: 11776477