Evaluation of hospital environment for presence of Mucorales during COVID-19-associated mucormycosis outbreak in India - a multi-centre study.

Air contamination COVID-19 Environmental contamination Epidemiology Mucormycosis Outbreak

Journal

The Journal of hospital infection
ISSN: 1532-2939
Titre abrégé: J Hosp Infect
Pays: England
ID NLM: 8007166

Informations de publication

Date de publication:
Apr 2022
Historique:
received: 07 11 2021
revised: 15 01 2022
accepted: 15 01 2022
pubmed: 7 2 2022
medline: 31 3 2022
entrez: 6 2 2022
Statut: ppublish

Résumé

An unprecedented rise in the number of COVID-19-associated mucormycosis (CAM) cases has been reported in India. Myriad hypotheses are proposed for the outbreak. We recently reported uncontrolled diabetes and inappropriate steroid therapy as significant risk factors for the outbreak. However, Mucorales contamination of hospital environment was not studied. To perform a multi-centre study across India to determine possible Mucorales contamination of hospital environment during the outbreak. Eleven hospitals from four zones of India representing high to low incidence for mucormycosis cases were included in the study. Samples from a variety of equipment used by the patients and ambient air were collected during May 19 None of the hospital equipment sampled was contaminated with Mucorales. However, Mucorales were isolated from 11.1% air-conditioning vents and 1.7% of patients' used masks. Other fungi were isolated from 18% of hospital equipment and surfaces, and 8.1% of used masks. Mucorales grew from 21.7% indoor and 53.8% outdoor air samples. Spore counts of Mucorales in air were significantly higher in the hospitals of North and South zones compared to West and East zones (P < 0.0001). Among Mucorales isolated from the environment, Rhizopus spp. were the most frequent genus. Contamination of air-conditioning vents and hospital air by Mucorales was found. Presence of Mucorales in these areas demands regular surveillance and improvement of hospital environment, as contamination may contribute to healthcare-associated mucormycosis outbreaks, especially among immunocompromised patients.

Sections du résumé

BACKGROUND BACKGROUND
An unprecedented rise in the number of COVID-19-associated mucormycosis (CAM) cases has been reported in India. Myriad hypotheses are proposed for the outbreak. We recently reported uncontrolled diabetes and inappropriate steroid therapy as significant risk factors for the outbreak. However, Mucorales contamination of hospital environment was not studied.
AIM OBJECTIVE
To perform a multi-centre study across India to determine possible Mucorales contamination of hospital environment during the outbreak.
METHODS METHODS
Eleven hospitals from four zones of India representing high to low incidence for mucormycosis cases were included in the study. Samples from a variety of equipment used by the patients and ambient air were collected during May 19
FINDINGS RESULTS
None of the hospital equipment sampled was contaminated with Mucorales. However, Mucorales were isolated from 11.1% air-conditioning vents and 1.7% of patients' used masks. Other fungi were isolated from 18% of hospital equipment and surfaces, and 8.1% of used masks. Mucorales grew from 21.7% indoor and 53.8% outdoor air samples. Spore counts of Mucorales in air were significantly higher in the hospitals of North and South zones compared to West and East zones (P < 0.0001). Among Mucorales isolated from the environment, Rhizopus spp. were the most frequent genus.
CONCLUSION CONCLUSIONS
Contamination of air-conditioning vents and hospital air by Mucorales was found. Presence of Mucorales in these areas demands regular surveillance and improvement of hospital environment, as contamination may contribute to healthcare-associated mucormycosis outbreaks, especially among immunocompromised patients.

Identifiants

pubmed: 35124141
pii: S0195-6701(22)00031-7
doi: 10.1016/j.jhin.2022.01.016
pmc: PMC8810519
pii:
doi:

Types de publication

Journal Article Multicenter Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

173-179

Informations de copyright

Copyright © 2022 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

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Auteurs

M Biswal (M)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

P Gupta (P)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

R Kanaujia (R)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

K Kaur (K)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

H Kaur (H)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

A Vyas (A)

Department of Microbiology, SMS Medical College, Jaipur, Rajasthan, India.

V Hallur (V)

All India Institute of Medical Sciences, Bhubaneswar, India.

B Behera (B)

All India Institute of Medical Sciences, Bhubaneswar, India.

P Padaki (P)

Department of Microbiology, St John's Medical College, Bengaluru, Karnataka, India.

J Savio (J)

Department of Microbiology, St John's Medical College, Bengaluru, Karnataka, India.

S Nagaraj (S)

Department of Microbiology, St John's Medical College, Bengaluru, Karnataka, India.

S K Chunchanur (SK)

Department of Microbiology, Bangalore Medical College and Research Institute, Bangalore, India.

J V Shwetha (JV)

Department of Microbiology, Bangalore Medical College and Research Institute, Bangalore, India.

R Ambica (R)

Department of Microbiology, Bangalore Medical College and Research Institute, Bangalore, India.

N Nagdeo (N)

Department of Microbiology, NKP Salve Institute of Medical Science and Research Centre and LMH, Nagpur, Maharashtra, India.

R Khuraijam (R)

Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India.

N Priyolakshmi (N)

Department of Microbiology, Regional Institute of Medical Sciences, Imphal, Manipur, India.

K Patel (K)

Sterling Hospital, Ahmedabad, Gujarat, India.

D Thamke (D)

Department of Microbiology, Mahatma Gandhi Institute of Medical Sciences, Wardha, Maharashtra, India.

L Dash (L)

Department of Microbiology, BYL Nair Ch. Hospital, Mumbai, India.

D Jadhav (D)

Department of Microbiology, BYL Nair Ch. Hospital, Mumbai, India.

R Bharmal (R)

Department of Microbiology, BYL Nair Ch. Hospital, Mumbai, India.

S Bhattacharya (S)

Tata Medical Center, Kolkata, India.

S M Rudramurthy (SM)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

A Chakrabarti (A)

Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. Electronic address: aurnaloke@hotmail.com.

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