The Probiotic Identity Card: A Novel "Probiogenomics" Approach to Investigate Probiotic Supplements.
cell viability
flow cytometry
genomics
metagenomics
probiotics
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
07
10
2021
accepted:
14
12
2021
entrez:
7
2
2022
pubmed:
8
2
2022
medline:
8
2
2022
Statut:
epublish
Résumé
Probiotic bacteria are widely administered as dietary supplements and incorporated as active ingredients in a variety of functional foods due to their purported health-promoting features. Currently available probiotic products may have issues with regards to their formulation, such as insufficient levels of viable probiotic bacteria, complete lack of probiotic strains that are stated to be present in the product, and the presence of microbial contaminants. To avoid the distribution of such unsuitable or misleading products, we propose here a novel approach named Probiotic Identity Card (PIC), involving a combination of shotgun metagenomic sequencing and bacterial cell enumeration by flow cytometry. PIC was tested on 12 commercial probiotic supplements revealing several inconsistencies in the formulation of five such products based on their stated microbial composition and viability.
Identifiants
pubmed: 35126330
doi: 10.3389/fmicb.2021.790881
pmc: PMC8814603
doi:
Types de publication
Journal Article
Langues
eng
Pagination
790881Informations de copyright
Copyright © 2022 Lugli, Longhi, Alessandri, Mancabelli, Tarracchini, Fontana, Turroni, Milani, Di Pierro, van Sinderen and Ventura.
Déclaration de conflit d'intérêts
GL and FF were employed by GenProbio Srl. FD was employed by company Velleja Research. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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