Regenerating the Injured Spinal Cord at the Chronic Phase by Engineered iPSCs-Derived 3D Neuronal Networks.
3D neuronal network
chronic phase injury
differentiation
induced pluripotent stem cells
spinal cord injury
tissue engineering
tissue implants
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
15
12
2021
pubmed:
8
2
2022
medline:
16
4
2022
entrez:
7
2
2022
Statut:
ppublish
Résumé
Cell therapy using induced pluripotent stem cell-derived neurons is considered a promising approach to regenerate the injured spinal cord (SC). However, the scar formed at the chronic phase is not a permissive microenvironment for cell or biomaterial engraftment or for tissue assembly. Engineering of a functional human neuronal network is now reported by mimicking the embryonic development of the SC in a 3D dynamic biomaterial-based microenvironment. Throughout the in vitro cultivation stage, the system's components have a synergistic effect, providing appropriate cues for SC neurogenesis. While the initial biomaterial supported efficient cell differentiation in 3D, the cells remodeled it to provide an inductive microenvironment for the assembly of functional SC implants. The engineered tissues are characterized for morphology and function, and their therapeutic potential is investigated, revealing improved structural and functional outcomes after acute and chronic SC injuries. Such technology is envisioned to be translated to the clinic to rewire human injured SC.
Identifiants
pubmed: 35128819
doi: 10.1002/advs.202105694
pmc: PMC9008789
doi:
Substances chimiques
Biocompatible Materials
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2105694Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
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