A bacterial endosymbiont of the fungus Rhizopus microsporus drives phagocyte evasion and opportunistic virulence.
Dictyostelium
Murcomycete
Ralstonia
Rhizopus
endosymbiosis
evolution
fungal pathogenesis
soil microbiology
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
14 03 2022
14 03 2022
Historique:
received:
23
11
2020
revised:
04
11
2021
accepted:
11
01
2022
pubmed:
9
2
2022
medline:
12
4
2022
entrez:
8
2
2022
Statut:
ppublish
Résumé
Opportunistic infections by environmental fungi are a growing clinical problem, driven by an increasing population of people with immunocompromising conditions. Spores of the Mucorales order are ubiquitous in the environment but can also cause acute invasive infections in humans through germination and evasion of the mammalian host immune system. How they achieve this and the evolutionary drivers underlying the acquisition of virulence mechanisms are poorly understood. Here, we show that a clinical isolate of Rhizopus microsporus contains a Ralstonia pickettii bacterial endosymbiont required for virulence in both zebrafish and mice and that this endosymbiosis enables the secretion of factors that potently suppress growth of the soil amoeba Dictyostelium discoideum, as well as their ability to engulf and kill other microbes. As amoebas are natural environmental predators of both bacteria and fungi, we propose that this tri-kingdom interaction contributes to establishing endosymbiosis and the acquisition of anti-phagocyte activity. Importantly, we show that this activity also protects fungal spores from phagocytosis and clearance by human macrophages, and endosymbiont removal renders the fungal spores avirulent in vivo. Together, these findings describe a new role for a bacterial endosymbiont in Rhizopus microsporus pathogenesis in animals and suggest a mechanism of virulence acquisition through environmental interactions with amoebas.
Identifiants
pubmed: 35134329
pii: S0960-9822(22)00039-2
doi: 10.1016/j.cub.2022.01.028
pmc: PMC8926845
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1115-1130.e6Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S010122/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 211241/Z/18/Z
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI063503
Pays : United States
Organisme : Wellcome Trust
ID : 102705/Z/13/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 108387/Z/15/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 097377
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V033417/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M01116X/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 102705
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N006364/2
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M014525/1
Pays : United Kingdom
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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