Magainin 2 and PGLa in bacterial membrane mimics III: Membrane fusion and disruption.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
01 03 2022
01 03 2022
Historique:
received:
20
09
2021
revised:
27
11
2021
accepted:
22
12
2021
pubmed:
9
2
2022
medline:
15
4
2022
entrez:
8
2
2022
Statut:
ppublish
Résumé
We previously speculated that the synergistically enhanced antimicrobial activity of Magainin 2 and PGLa is related to membrane adhesion, fusion, and further membrane remodeling. Here we combined computer simulations with time-resolved in vitro fluorescence microscopy, cryoelectron microscopy, and small-angle X-ray scattering to interrogate such morphological and topological changes of vesicles at nanoscopic and microscopic length scales in real time. Coarse-grained simulations revealed formation of an elongated and bent fusion zone between vesicles in the presence of equimolar peptide mixtures. Vesicle adhesion and fusion were observed to occur within a few seconds by cryoelectron microscopy and corroborated by small-angle X-ray scattering measurements. The latter experiments indicated continued and time-extended structural remodeling for individual peptides or chemically linked peptide heterodimers but with different kinetics. Fluorescence microscopy further captured peptide-dependent adhesion, fusion, and occasional bursting of giant unilamellar vesicles a few seconds after peptide addition. The synergistic interactions between the peptides shorten the time response of vesicles and enhance membrane fusogenic and disruption properties of the equimolar mixture compared with the individual peptides.
Identifiants
pubmed: 35134334
pii: S0006-3495(21)03959-X
doi: 10.1016/j.bpj.2021.12.035
pmc: PMC8943694
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Magainins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
852-861Informations de copyright
Copyright © 2022 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Références
J Chem Theory Comput. 2008 May;4(5):819-34
pubmed: 26621095
Biochemistry. 2007 Dec 11;46(49):14284-90
pubmed: 18004888
J Phys Condens Matter. 2006 Jul 19;18(28):S1151-76
pubmed: 21690835
Front Med Technol. 2021 Feb 24;3:625975
pubmed: 35047906
Biophys J. 2013 Jul 2;105(1):154-64
pubmed: 23823234
Lab Chip. 2019 Feb 12;19(4):626-633
pubmed: 30632596
J Chem Theory Comput. 2016 Jan 12;12(1):405-13
pubmed: 26631602
Sci Rep. 2017 Oct 13;7(1):13153
pubmed: 29030606
Biochim Biophys Acta. 2005 Oct 1;1716(1):40-8
pubmed: 16150420
Biophys J. 2019 Nov 19;117(10):1858-1869
pubmed: 31703802
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):12883-8
pubmed: 20566852
Biochemistry. 1998 Oct 27;37(43):15144-53
pubmed: 9790678
Biophys J. 2018 Sep 18;115(6):1033-1044
pubmed: 30195937
J Chem Phys. 2007 Jan 7;126(1):014101
pubmed: 17212484
Chembiochem. 2010 May 3;11(7):848-65
pubmed: 20336703
Biophys J. 2013 Mar 19;104(6):L9-11
pubmed: 23528099
Integr Biol (Camb). 2012 Sep;4(9):982-95
pubmed: 22829218
Biophys J. 2018 Apr 24;114(8):1945-1954
pubmed: 29694871
J Synchrotron Radiat. 1998 May 1;5(Pt 3):506-8
pubmed: 15263560
Annu Rev Biophys. 2019 May 6;48:93-119
pubmed: 30811220
J Biol Chem. 1959 Mar;234(3):466-8
pubmed: 13641241
Biochemistry. 1989 May 2;28(9):3692-703
pubmed: 2751990
Sci Rep. 2020 Jul 15;10(1):11652
pubmed: 32669585
Langmuir. 2014 Sep 2;30(34):10374-83
pubmed: 25105913
Biophys J. 2020 Feb 4;118(3):612-623
pubmed: 31952806
J Chem Theory Comput. 2013 Jan 8;9(1):687-97
pubmed: 26589065
J Phys Chem B. 2007 Jul 12;111(27):7812-24
pubmed: 17569554