Physiological synaptic activity and recognition memory require astroglial glutamine.

Animals Astrocytes / metabolism Cognition Fluorescent Dyes / chemistry Glutamic Acid / chemistry Glutamine / chemistry Hippocampus / cytology Intravital Microscopy Male Mice Mice, Transgenic Models, Animal Molecular Probes Neurons / metabolism Recognition, Psychology Rhodamines / chemistry Stereotaxic Techniques Synaptic Transmission

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
08 02 2022
Historique:
received: 05 10 2020
accepted: 07 01 2022
entrez: 9 2 2022
pubmed: 10 2 2022
medline: 5 3 2022
Statut: epublish

Résumé

Presynaptic glutamate replenishment is fundamental to brain function. In high activity regimes, such as epileptic episodes, this process is thought to rely on the glutamate-glutamine cycle between neurons and astrocytes. However the presence of an astroglial glutamine supply, as well as its functional relevance in vivo in the healthy brain remain controversial, partly due to a lack of tools that can directly examine glutamine transfer. Here, we generated a fluorescent probe that tracks glutamine in live cells, which provides direct visual evidence of an activity-dependent glutamine supply from astroglial networks to presynaptic structures under physiological conditions. This mobilization is mediated by connexin43, an astroglial protein with both gap-junction and hemichannel functions, and is essential for synaptic transmission and object recognition memory. Our findings uncover an indispensable recruitment of astroglial glutamine in physiological synaptic activity and memory via an unconventional pathway, thus providing an astrocyte basis for cognitive processes.

Identifiants

DOI: 10.1038/s41467-022-28331-7 PMID: 35136061 PMC: PMC8826940
pubmed: 35136061
doi: 10.1038/s41467-022-28331-7
pii: 10.1038/s41467-022-28331-7
pmc: PMC8826940
doi:

Substances chimiques

Fluorescent Dyes 0
Molecular Probes 0
Rhodamines 0
Glutamine 0RH81L854J
Glutamic Acid 3KX376GY7L

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

753

Commentaires et corrections

Type : CommentIn

Informations de copyright

© 2022. The Author(s).

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Auteurs

Giselle Cheung (G)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Institute of Science and Technology Austria, Klosterneuburg, Austria.
ORCID: 0000-0001-8457-2572

Danijela Bataveljic (D)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Institute for Physiology and Biochemistry Ivan Djaja, Faculty of Biology, University of Belgrade, Belgrade, Serbia.
ORCID: 0000-0002-9593-8231

Josien Visser (J)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Doctoral School N°158, Pierre and Marie Curie University, Paris, France.

Naresh Kumar (N)

Université Paris Saclay, ENS Paris-Saclay, CNRS, PPSM, 91190, Gif-sur-Yvette, France.
ORCID: 0000-0003-0170-888X

Julien Moulard (J)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Doctoral School N°158, Pierre and Marie Curie University, Paris, France.
ORCID: 0000-0002-6075-9081

Glenn Dallérac (G)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Université Paris-Saclay, CNRS, Institut des Neurosciences Paris-Saclay, Gif-sur-Yvette, France.
ORCID: 0000-0002-4518-4099

Daria Mozheiko (D)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Doctoral School N°158, Pierre and Marie Curie University, Paris, France.

Astrid Rollenhagen (A)

Institute for Neuroscience and Medicine INM-10, Research Center Jülich, Jülich, Germany.
Department of Psychiatry, Psychotherapy and Psychosomatics, Rheinisch-Westfaelische Technische Hochschule Aachen University, Aachen, Germany.

Pascal Ezan (P)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.

Cédric Mongin (C)

Université Paris Saclay, ENS Paris-Saclay, CNRS, PPSM, 91190, Gif-sur-Yvette, France.
ORCID: 0000-0002-0565-0606

Oana Chever (O)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France.
Normandy University, UNIROUEN, INSERM, DC2N, 76000, Rouen, France.
ORCID: 0000-0003-3081-1798

Alexis-Pierre Bemelmans (AP)

Commissariat à l'Energie Atomique et aux Energies Alternatives, Département de la Recherche Fondamentale, Institut de biologie François Jacob, Molecular Imaging Research Center and CNRS UMR9199, Université Paris-Sud, Neurodegenerative Diseases Laboratory, Fontenay-aux-Roses, France.
ORCID: 0000-0001-7605-5225

Joachim Lübke (J)

Institute for Neuroscience and Medicine INM-10, Research Center Jülich, Jülich, Germany.
Department of Psychiatry, Psychotherapy and Psychosomatics, Rheinisch-Westfaelische Technische Hochschule Aachen University, Aachen, Germany.
Jülich-Aachen Research Alliance Translational Brain Medicine, Aachen, Germany.

Isabelle Leray (I)

Université Paris Saclay, ENS Paris-Saclay, CNRS, PPSM, 91190, Gif-sur-Yvette, France.

Nathalie Rouach (N)

Neuroglial Interactions in Cerebral Physiology and Pathologies, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS, INSERM, Labex Memolife, Université PSL, Paris, France. nathalie.rouach@college-de-france.fr.
ORCID: 0000-0002-5574-888X

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questionsmedicales.fr › Eucaryotes › Animaux › Physiological synaptic activity and...
questionsmedicales.fr › Cellules › Névroglie › Astrocytes › Physiological synaptic activity and...
questionsmedicales.fr › Phénomènes psychologiques › Processus mentaux › Cognition › Physiological synaptic activity and...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Physiological synaptic activity and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés excitateurs › Acide glutamique › Physiological synaptic activity and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés neutres › Glutamine › Physiological synaptic activity and...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Prosencéphale › Télencéphale › Cerveau › Cortex cérébral › Hippocampe › Physiological synaptic activity and...
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