Droplet digital PCR assay provides intrahepatic HBV cccDNA quantification tool for clinical application.
Animals
Carcinoma, Hepatocellular
/ virology
DNA, Circular
/ analysis
DNA, Viral
/ analysis
Hepatitis B
/ drug therapy
Hepatitis B virus
/ genetics
Humans
Interferon-alpha
/ therapeutic use
Liver
/ virology
Mice, Transgenic
Polyethylene Glycols
/ therapeutic use
Polymerase Chain Reaction
/ methods
Recombinant Proteins
/ therapeutic use
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 02 2022
08 02 2022
Historique:
received:
05
10
2021
accepted:
13
01
2022
entrez:
9
2
2022
pubmed:
10
2
2022
medline:
16
3
2022
Statut:
epublish
Résumé
The persistence of covalently closed circular DNA (cccDNA) poses a major obstacle to curing chronic hepatitis B (CHB). Here, we used droplet digital PCR (ddPCR) for cccDNA quantitation. The cccDNA-specific ddPCR showed high accuracy with the dynamic range of cccDNA detection from 10
Identifiants
pubmed: 35136096
doi: 10.1038/s41598-022-05882-9
pii: 10.1038/s41598-022-05882-9
pmc: PMC8826402
doi:
Substances chimiques
DNA, Circular
0
DNA, Viral
0
Interferon-alpha
0
Recombinant Proteins
0
Polyethylene Glycols
3WJQ0SDW1A
peginterferon alfa-2a
Q46947FE7K
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
2133Informations de copyright
© 2022. The Author(s).
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