Alkylated glutamic acid and histidine derived from protein-adducts indicate exposure to sulfur mustard in avian serum.
Organisation for the Prohibition of Chemical Weapons
alkylation
mass spectrometry
protein-adducts
vesicants
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
revised:
01
02
2022
received:
13
01
2022
accepted:
03
02
2022
pubmed:
10
2
2022
medline:
15
6
2022
entrez:
9
2
2022
Statut:
ppublish
Résumé
Sulfur mustard (SM, bis[2-chloroethyl]-sulfide) is a banned chemical warfare agent deployed in the violent conflict in the Middle East poisoning humans and animals. For legal reasons, bioanalytical methods are mandatory proving exposure to SM. Reaction products (adducts) of SM with endogenous proteins, for example, serum albumin (SA), are valuable long-lived targets for analysis. Whereas nearly all methods known so far focus on human proteins, we address for the first time neat chicken SA and avian serum from chicken, duck, and ostrich. After proteolysis, protein precipitation, evaporation of the supernatant, and re-dissolution analysis were performed by micro-liquid chromatography-electrospray ionization tandem-mass spectrometry in the selected reaction monitoring mode, μLC-ESI MS/MS (SRM), for detection of the hydroxyethylthioethyl product ion [HETE]
Substances chimiques
Chemical Warfare Agents
0
Hydroxyeicosatetraenoic Acids
0
Serum Albumin
0
Glutamic Acid
3KX376GY7L
Histidine
4QD397987E
Mustard Gas
T8KEC9FH9P
Serum Albumin, Human
ZIF514RVZR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1140-1148Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : Research Training Group GRK 2338
Informations de copyright
© 2022 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
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