Chemokine production induced by Corynebacterium pseudotuberculosis in a murine model.
Caseous lymphadenitis
Chemotaxis
Corynebacteriaceae
Granuloma
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
14
06
2021
accepted:
01
02
2022
pubmed:
10
2
2022
medline:
3
6
2022
entrez:
9
2
2022
Statut:
ppublish
Résumé
Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis. The main clinical sign of this disease is the development of granulomas, especially in small ruminants; however, the pathways that are involved in the formation and maintenance of these granulomas are unknown. Cytokines and chemokines are responsible for the migration of immune cells to specific sites and tissues; therefore, it is possible that chemokines participate in abscess formation. This study aimed to evaluate the induction of chemokine production by two C. pseudotuberculosis strains in a murine model. A highly pathogenic (VD57) and an attenuated (T1) strain of C. pseudotuberculosis, as well as somatic and secreted antigens derived from these strains, was used to stimulate murine splenocytes. Then, the concentrations of the chemokines CCL-2, CCL-3, CCL-4, and CCL-5 and the cytokines IL-1 and TNF were measured in the culture supernatants. The VD57 strain had a higher ability to stimulate the production of chemokines when compared to T1 strain, especially in the early stages of stimulation, which can have an impact on granuloma formation. The T1 lysate antigen was able to stimulate most of the chemokines studied herein when compared to the other antigenic fractions of both strains. These results indicate that C. pseudotuberculosis is a chemokine production inducer, and the bacterial strains differ in their induction pattern, a situation that can be related to the specific behavior of each strain.
Identifiants
pubmed: 35138630
doi: 10.1007/s42770-022-00694-5
pii: 10.1007/s42770-022-00694-5
pmc: PMC9151972
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1019-1027Subventions
Organisme : fapesb
ID : 022/2009
Organisme : conselho nacional de desenvolvimento científico e tecnológico
ID : 313350/2019-1
Informations de copyright
© 2022. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
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