An optogenetic approach for regulating human parathyroid hormone secretion.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 02 2022
09 02 2022
Historique:
received:
21
09
2019
accepted:
25
01
2022
entrez:
10
2
2022
pubmed:
11
2
2022
medline:
8
3
2022
Statut:
epublish
Résumé
Parathyroid hormone (PTH) plays crucial role in maintaining calcium and phosphorus homeostasis. In the progression of secondary hyperparathyroidism (SHPT), expression of calcium-sensing receptors (CaSR) in the parathyroid gland decreases, which leads to persistent hypersecretion of PTH. How to precisely manipulate PTH secretion in parathyroid tissue and underlying molecular mechanism is not clear. Here, we establish an optogenetic approach that bypasses CaSR to inhibit PTH secretion in human hyperplastic parathyroid cells. We found that optogenetic stimulation elevates intracellular calcium, inhibits both PTH synthesis and secretion in human parathyroid cells. Long-term pulsatile PTH secretion induced by light stimulation prevented hyperplastic parathyroid tissue-induced bone loss by influencing the bone remodeling in mice. The effects are mediated by light stimulation of opsin expressing parathyroid cells and other type of cells in parathyroid tissue. Our study provides a strategy to regulate release of PTH and associated bone loss of SHPT through an optogenetic approach.
Identifiants
pubmed: 35140213
doi: 10.1038/s41467-022-28472-9
pii: 10.1038/s41467-022-28472-9
pmc: PMC8828854
doi:
Substances chimiques
Parathyroid Hormone
0
Receptors, Calcium-Sensing
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
771Informations de copyright
© 2022. The Author(s).
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