Functional characterization of human organic anion transporter 10 (OAT10/SLC22A13) as an orotate transporter.
Animal species difference
Kidney
Nicotinate
OAT10
Orotate
Reabsorption
Journal
Drug metabolism and pharmacokinetics
ISSN: 1880-0920
Titre abrégé: Drug Metab Pharmacokinet
Pays: England
ID NLM: 101164773
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
28
09
2021
revised:
07
12
2021
accepted:
17
12
2021
pubmed:
11
2
2022
medline:
17
3
2022
entrez:
10
2
2022
Statut:
ppublish
Résumé
Orotate, a nutritional compound typically utilized as an intermediate in pyrimidine synthesis, has been suggested to undergo renal reabsorption. However, the detailed mechanisms involved in the process remain unclear, with only urate transporter 1 (URAT1/SLC22A12) being indicated as a transporter involved in its tubular uptake. As an attempt to identify transporters involved in that to help clarify the mechanisms, we examined a possibility that organic anion transporter 10 (OAT10/SLC22A13), which is present at the brush border membrane in renal tubular epithelial cells, could transport orotate. The operation of human OAT10 for orotate transport was demonstrated indeed and analyzed in detail in Madin-Darby canine kidney II cells introduced with this transporter by stable transfection. Orotate transport by OAT10 was found to be kinetically saturable with a biphasic characteristic and dependent on Cl
Identifiants
pubmed: 35144162
pii: S1347-4367(21)00064-1
doi: 10.1016/j.dmpk.2021.100443
pii:
doi:
Substances chimiques
Organic Anion Transporters
0
Organic Cation Transport Proteins
0
SLC22A12 protein, human
0
SLC22A13 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
100443Informations de copyright
Copyright © 2021 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.