Exercise Increases Bone in SEIPIN Deficient Lipodystrophy, Despite Low Marrow Adiposity.


Journal

Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782

Informations de publication

Date de publication:
2021
Historique:
received: 27 09 2021
accepted: 20 12 2021
entrez: 11 2 2022
pubmed: 12 2 2022
medline: 3 3 2022
Statut: epublish

Résumé

Exercise, typically beneficial for skeletal health, has not yet been studied in lipodystrophy, a condition characterized by paucity of white adipose tissue, with eventual diabetes, and steatosis. We applied a mouse model of global deficiency of Bscl2 (SEIPIN), required for lipid droplet formation. Male twelve-week-old B6 knockouts (KO) and wild type (WT) littermates were assigned six-weeks of voluntary, running exercise (E) versus non-exercise (N=5-8). KO weighed 14% less than WT (p=0.01) and exhibited an absence of epididymal adipose tissue; KO liver Plin1 via qPCR was 9-fold that of WT (p=0.04), consistent with steatosis. Bone marrow adipose tissue (BMAT), unlike white adipose, was measurable, although 40.5% lower in KO vs WT (p=0.0003) via 9.4T MRI/advanced image analysis. SEIPIN ablation's most notable effect marrow adiposity was in the proximal femoral diaphysis (-56% KO vs WT, p=0.005), with relative preservation in KO-distal-femur. Bone via μCT was preserved in SEIPIN KO, though some quality parameters were attenuated. Running distance, speed, and time were comparable in KO and WT. Exercise reduced weight (-24% WT-E vs WT p<0.001) but not in KO. Notably, exercise increased trabecular BV/TV in both (+31%, KO-E vs KO, p=0.004; +14%, WT-E vs WT, p=0.006). The presence and distribution of BMAT in SEIPIN KO, though lower than WT, is unexpected and points to a uniqueness of this depot. That trabecular bone increases were achievable in both KO and WT, despite a difference in BMAT quantity/distribution, points to potential metabolic flexibility during exercise-induced skeletal anabolism.

Identifiants

pubmed: 35145475
doi: 10.3389/fendo.2021.782194
pmc: PMC8822583
doi:

Substances chimiques

Bscl2 protein, mouse 0
GTP-Binding Protein gamma Subunits 0
Perilipin-1 0
Plin1 protein, mouse 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

782194

Subventions

Organisme : NIAMS NIH HHS
ID : R01 AR073264
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR075803
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK107481
Pays : United States
Organisme : NCATS NIH HHS
ID : KL2 TR002490
Pays : United States

Informations de copyright

Copyright © 2022 McGrath, Little-Letsinger, Sankaran, Sen, Xie, Styner, Zong, Chen, Rubin, Klett, Coleman and Styner.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

J Clin Invest. 2004 Mar;113(6):846-55
pubmed: 15067317
J Clin Endocrinol Metab. 2003 Nov;88(11):5433-7
pubmed: 14602785
J Bone Miner Res. 2020 Jan;35(1):106-115
pubmed: 31509274
Mol Cell Biol. 2016 Jul 14;36(15):2027-38
pubmed: 27185876
N Engl J Med. 2004 Mar 18;350(12):1220-34
pubmed: 15028826
J Cell Biochem. 2010 Nov 1;111(4):1042-50
pubmed: 20672310
J Clin Endocrinol Metab. 2008 Apr;93(4):1129-34
pubmed: 18211975
J Musculoskelet Neuronal Interact. 2007 Jan-Mar;7(1):84-93
pubmed: 17396014
Neuroimage. 2004;23 Suppl 1:S151-60
pubmed: 15501084
JBMR Plus. 2020 Sep 08;4(10):e10400
pubmed: 33103029
Bone. 2020 Jul;136:115362
pubmed: 32289518
Nat Commun. 2020 Jun 18;11(1):3097
pubmed: 32555194
J Clin Densitom. 2015 Apr-Jun;18(2):203-8
pubmed: 25840474
Bone. 2012 Sep;51(3):570-7
pubmed: 22584009
Endocrinology. 2015 Aug;156(8):2753-61
pubmed: 26052898
J Clin Endocrinol Metab. 2012 Apr;97(4):1337-46
pubmed: 22319043
Dev Cell. 2012 Jul 17;23(1):71-81
pubmed: 22814601
Acta Paediatr Suppl. 1996 Jun;413:44-51
pubmed: 8783772
J Clin Endocrinol Metab. 2010 Jan;95(1):407-13
pubmed: 19850693
Mol Cell Biol. 2012 Mar;32(6):1099-111
pubmed: 22269949
Curr Osteoporos Rep. 2020 Dec;18(6):774-789
pubmed: 33068251
J Exp Biol. 2015 Oct;218(Pt 19):3002-9
pubmed: 26232415
J Appl Physiol (1985). 2009 Aug;107(2):540-8
pubmed: 19478189
Mol Metab. 2018 Apr;10:55-65
pubmed: 29459250
Bone. 2015 Oct;79:203-12
pubmed: 26092649
Exp Mol Med. 2003 Dec 31;35(6):518-26
pubmed: 14749529
Magn Reson Imaging. 2012 Nov;30(9):1323-41
pubmed: 22770690
J Ind Microbiol Biotechnol. 2019 Dec;46(12):1769-1780
pubmed: 31541399
Exerc Sport Sci Rev. 2018 Jan;46(1):42-47
pubmed: 28795956
Nat Med. 2013 May;19(5):635-9
pubmed: 23603815
Nat Genet. 2001 Aug;28(4):365-70
pubmed: 11479539
J Bone Miner Res. 2003 Nov;18(11):2002-9
pubmed: 14606513
Bone. 2017 Jun;99:69-79
pubmed: 28366796
Med Eng Phys. 2009 Jan;31(1):34-41
pubmed: 18486521
Cell Rep. 2016 Nov 1;17(6):1546-1559
pubmed: 27806294
Cell. 2000 Jan 21;100(2):197-207
pubmed: 10660043
FASEB J. 2002 Aug;16(10):1280-2
pubmed: 12153999
Skeletal Radiol. 2007 Oct;36(10):999-1003
pubmed: 17554536
J Bone Joint Surg Am. 1977 Mar;59(2):204-8
pubmed: 845205
Front Endocrinol (Lausanne). 2020 Sep 23;11:578194
pubmed: 33071983
Bone. 2008 Sep;43(3):613-20
pubmed: 18567552
Bone. 2013 Jan;52(1):454-64
pubmed: 22951341
Radiology. 1990 Apr;175(1):219-23
pubmed: 2315484
Nat Rev Endocrinol. 2019 Jun;15(6):339-355
pubmed: 30814687
J Clin Densitom. 2018 Jan - Mar;21(1):61-67
pubmed: 27894728
J Bone Miner Res. 2018 Dec;33(12):2158-2164
pubmed: 30075054
Sci Rep. 2016 Oct 17;6:35487
pubmed: 27748422
Bone. 2016 Sep;90:69-79
pubmed: 27262776
J Bone Miner Res. 2018 Feb;33(2):211-220
pubmed: 28975661
J Clin Endocrinol Metab. 1992 Aug;75(2):358-61
pubmed: 1639935
Clin Endocrinol (Oxf). 2009 Oct;71(4):512-7
pubmed: 19226263
Endocrinology. 2014 Nov;155(11):4215-25
pubmed: 25093462
J Clin Endocrinol Metab. 2002 May;87(5):2395
pubmed: 11994394
Front Endocrinol (Lausanne). 2016 Jul 27;7:102
pubmed: 27512386
J Bone Miner Res. 2007 Sep;22(9):1408-18
pubmed: 17550335
Proc Natl Acad Sci U S A. 2014 May 13;111(19):7054-9
pubmed: 24778225
JCI Insight. 2017 Aug 17;2(16):
pubmed: 28814665
Nat Rev Endocrinol. 2015 Sep;11(9):522-34
pubmed: 26239609
Am J Med. 2009 May;122(5):409-14
pubmed: 19375545
J Bone Miner Res. 2021 Apr;36(4):673-684
pubmed: 33314313
Bone. 2020 Sep;138:115496
pubmed: 32585320
J Bone Miner Res. 1995 Apr;10(4):574-85
pubmed: 7610928
J Clin Endocrinol Metab. 1954 Feb;14(2):193-204
pubmed: 13130666
Acta Paediatr. 1959 Nov;48:555-74
pubmed: 14444642
J Clin Endocrinol Metab. 2016 Dec;101(12):4500-4511
pubmed: 27710244
Annu Rev Nutr. 2014;34:1-30
pubmed: 24819326
Recent Prog Horm Res. 2002;57:385-409
pubmed: 12017554
J Biol Chem. 2009 Dec 11;284(50):34607-17
pubmed: 19840939
J Bone Miner Res. 2019 Oct;34(10):1815-1823
pubmed: 31216081
J Bone Miner Res. 2018 Apr;33(4):651-657
pubmed: 29336053
Mol Metab. 2020 Jun;36:100971
pubmed: 32246911
Diabetes Care. 2020 Oct;43(10):e149-e151
pubmed: 32801128
Diabetes Metab Res Rev. 2007 Nov;23(8):600-11
pubmed: 17966120
Acta Paediatr Suppl. 1996 Jun;413:2-28
pubmed: 8783769
Cell. 2002 Nov 1;111(3):305-17
pubmed: 12419242
Stem Cells. 2015 Jun;33(6):2063-76
pubmed: 25787126
Neuroimage. 2011 Feb 1;54(3):2033-44
pubmed: 20851191
Nature. 2020 Mar;579(7797):111-117
pubmed: 32103177
J Biol Chem. 2019 May 31;294(22):8819-8833
pubmed: 30975900
Proc Natl Acad Sci U S A. 2007 Nov 6;104(45):17879-84
pubmed: 17959771
Endocrinology. 2016 Feb;157(2):508-21
pubmed: 26696121
Bone. 2017 Aug;101:21-25
pubmed: 28390904
J Clin Endocrinol Metab. 2011 Nov;96(11):3313-25
pubmed: 21865368
Endocrinology. 2011 Jul;152(7):2655-64
pubmed: 21586558
J Clin Endocrinol Metab. 1999 Jan;84(1):170-4
pubmed: 9920078
Bone. 2009 Jun;44(6):1092-6
pubmed: 19268721
Neuroimage. 2006 Jul 1;31(3):1116-28
pubmed: 16545965
Circulation. 2004 Jan 20;109(2):220-6
pubmed: 14691039
J Bone Miner Res. 2020 Jun;35(6):1031-1039
pubmed: 32078187
J Bone Miner Res. 2020 Jun;35(6):1149-1162
pubmed: 32022326
Nat Rev Rheumatol. 2010 Jan;6(1):50-9
pubmed: 20046206
Skeletal Radiol. 1992;21(6):381-6
pubmed: 1523434
EMBO J. 2016 Dec 15;35(24):2699-2716
pubmed: 27879284
Endocr Pract. 2007 Nov-Dec;13(7):764-9
pubmed: 18194934
Ann Biomed Eng. 2003 Jan;31(1):12-20
pubmed: 12572652
PLoS One. 2012;7(12):e51613
pubmed: 23251594
Med Hypotheses. 2016 Dec;97:94-97
pubmed: 27876139
Hum Mol Genet. 2011 Aug 1;20(15):3022-30
pubmed: 21551454
Front Endocrinol (Lausanne). 2016 Jun 30;7:80
pubmed: 27445983
J Bone Miner Res. 2017 Aug;32(8):1692-1702
pubmed: 28436105
Lancet. 1971 Jul 10;2(7715):104-5
pubmed: 4103971
J Orthop Res. 1995 Sep;13(5):708-14
pubmed: 7472749
Bone. 2014 Jul;64:39-46
pubmed: 24709686
BMJ Open. 2017 Jun 12;7(6):e014951
pubmed: 28611110

Auteurs

Cody McGrath (C)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Sarah E Little-Letsinger (SE)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Jeyantt Srinivas Sankaran (JS)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Buer Sen (B)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Zhihui Xie (Z)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Martin A Styner (MA)

Department of Psychiatry, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
Department of Computer Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Xiaopeng Zong (X)

Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.

Weiqin Chen (W)

Department of Physiology, Medical College of Georgia at Augusta University, Augusta, GA, United States.

Janet Rubin (J)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
North Carolina Diabetes Research Center (NCDRC), Chapel Hill, NC, United States.

Eric L Klett (EL)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
North Carolina Diabetes Research Center (NCDRC), Chapel Hill, NC, United States.
Department of Nutrition, Gillings School of Global Public Health, UNC, Chapel Hill, NC, United States.

Rosalind A Coleman (RA)

Department of Nutrition, Gillings School of Global Public Health, UNC, Chapel Hill, NC, United States.

Maya Styner (M)

Department of Medicine, Division of Endocrinology & Metabolism, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.
North Carolina Diabetes Research Center (NCDRC), Chapel Hill, NC, United States.

Articles similaires

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male
Humans Meals Time Factors Female Adult

Classifications MeSH