Protocol for influenza A virus infection of mice and viral load determination.
Flow Cytometry/Mass Cytometry
Immunology
Microbiology
Microscopy
Model Organisms
Molecular Biology
Protein Biochemistry
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 03 2022
18 03 2022
Historique:
entrez:
11
2
2022
pubmed:
12
2
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Influenza A viruses (IAVs) are common respiratory viruses. Mouse models of IAV infection are valuable to study the mechanisms of IAV infection and pathology. Here, we present a detailed protocol for IAV infection of mice via intranasal administration. We detail the processing of mouse lung tissue and then describe the determination of viral load by several approaches including RNA, protein, or plaque-forming unit assays. This protocol may be adapted to other influenza strains or respiratory viruses. For complete details on the use and execution of this protocol, please refer to Galani et al. (2017).
Identifiants
pubmed: 35146450
doi: 10.1016/j.xpro.2022.101151
pii: S2666-1667(22)00031-4
pmc: PMC8819391
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101151Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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