Use of Fluorescence Recovery After Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction-Associated Polarity Proteins.
Drosophila
FRAP
Fluorescence
In vivo imaging
Live-imaging
PCP
Photobleaching
Planar cell polarity
Planar polarity
Pupal wing
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
11
2
2022
pubmed:
12
2
2022
medline:
19
2
2022
Statut:
ppublish
Résumé
Here, we present a detailed protocol for fluorescence recovery after photobleaching (FRAP) to measure the dynamics of junctional populations of proteins in living tissue. Specifically, we describe how to perform FRAP in Drosophila pupal wings on fluorescently tagged core planar polarity proteins, which exhibit relatively slow junctional turnover. We provide a step-by-step practical guide to performing FRAP, and list a series of controls and optimizations to do before conducting a FRAP experiment. Finally, we describe how to present the FRAP data for publication.
Identifiants
pubmed: 35147932
doi: 10.1007/978-1-0716-2035-9_1
doi:
Substances chimiques
Membrane Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-30Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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