Multiplexed flow cytometric approach for detection of anti-SARS-CoV-2 IgG, IgM and IgA using beads covalently coupled to the nucleocapsid protein.
CBA functional beads
COVID-19
IgG
IgM and IgA antibodies
SARS-CoV-2
flow cytometry
multiplex immunoassay
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
revised:
11
01
2022
received:
28
06
2021
accepted:
13
01
2022
pubmed:
12
2
2022
medline:
21
5
2022
entrez:
11
2
2022
Statut:
ppublish
Résumé
Flow cytometry has emerged as a promising technique for detection of SARS-CoV-2 antibodies. In this study, we developed an innovative strategy for simultaneous detection of immunoglobulin G (IgG), IgM and IgA. The SARS-CoV-2 nucleocapsid protein was covalently bound to functional beads surface applying sulpho-SMCC chemistry. BUV395 anti-IgG, BB515 anti-IgM, biotinylated anti-IgA1/IgA2 and BV421 streptavidin were used as fluorophore conjugated secondary antibodies. Serum and antibodies reaction conditions were optimized for each antibody isotype detection and a multiplexed detection assay was developed. This new cell-free assay efficiently discriminate COVID-19 negative and positive samples. The simultaneous detection of IgG, IgM and IgA showed a sensitivity of 88·5-96·2% and specificity of 100%. This novel strategy opens a new avenue for flow cytometry-based diagnosis.
Identifiants
pubmed: 35148433
doi: 10.1111/lam.13674
pmc: PMC9115257
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin A
0
Immunoglobulin G
0
Immunoglobulin M
0
Nucleocapsid Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
863-872Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 400953/2016-1
Informations de copyright
© 2022 The Society for Applied Microbiology.
Références
Eur Respir J. 2020 Aug 27;56(2):
pubmed: 32398307
Cell Death Differ. 2021 Feb;28(2):626-639
pubmed: 33479399
Nat Commun. 2021 Jan 4;12(1):6
pubmed: 33397903
Commun Biol. 2021 Apr 20;4(1):486
pubmed: 33879833
Transfusion. 2021 May;61(5):1377-1382
pubmed: 33604922
Clin Chim Acta. 2020 Aug;507:164-166
pubmed: 32343948
Nat Med. 2020 Jun;26(6):845-848
pubmed: 32350462
Virus Res. 2014 Dec 19;194:175-83
pubmed: 24670324
J Virol Methods. 2021 May;291:114111
pubmed: 33640374
ACS Sens. 2021 Mar 26;6(3):703-708
pubmed: 33496577
Front Immunol. 2020 Nov 30;11:611337
pubmed: 33329607
Commun Biol. 2021 Jan 29;4(1):129
pubmed: 33514825
J Biomed Sci. 2020 Jun 4;27(1):72
pubmed: 32498686
Eur J Immunol. 2021 Nov;51(11):2633-2640
pubmed: 34358329
Brain Behav Immun. 2020 Jul;87:182-183
pubmed: 32454136
J Infect Dis. 2004 Jul 15;190(2):379-86
pubmed: 15216476
J Infect Dis. 2020 Oct 1;222(9):1452-1461
pubmed: 32766833
Nat Immunol. 2020 Oct;21(10):1293-1301
pubmed: 32807944
Cytotechnology. 2022 Feb;74(1):99-103
pubmed: 35002070
Sci Transl Med. 2021 Jan 20;13(577):
pubmed: 33288662
Diagnostics (Basel). 2020 Apr 05;10(4):
pubmed: 32260471
Sci Rep. 2021 Feb 10;11(1):3461
pubmed: 33568715
Nat Commun. 2021 Feb 2;12(1):740
pubmed: 33531472
Viruses. 2021 Apr 24;13(5):
pubmed: 33923338
Sci Rep. 2021 Mar 23;11(1):6614
pubmed: 33758278
J Virol Methods. 2021 Feb;288:114025
pubmed: 33227340
Int J Infect Dis. 2020 Dec;101:382-390
pubmed: 33039612
Biointerphases. 2017 Mar 16;12(2):02D301
pubmed: 28301944
Signal Transduct Target Ther. 2021 Jan 29;6(1):35
pubmed: 33514692
Anal Bioanal Chem. 2021 Sep;413(22):5619-5632
pubmed: 33983466
Sci Transl Med. 2020 Sep 2;12(559):
pubmed: 32817357
EMBO Mol Med. 2021 Mar 5;13(3):e13549
pubmed: 33471406
Nat Rev Microbiol. 2021 Mar;19(3):141-154
pubmed: 33024307
Am J Emerg Med. 2021 Feb;40:217-219
pubmed: 32513451
Sci Rep. 2020 Nov 16;10(1):19893
pubmed: 33199713
Cell Rep Med. 2021 Feb 16;2(2):100193
pubmed: 33495757
Lancet. 2020 Apr 4;395(10230):1101-1102
pubmed: 32247384
Lancet Infect Dis. 2020 May;20(5):565-574
pubmed: 32213337
Emerg Infect Dis. 2020 Jul;26(7):1478-1488
pubmed: 32267220
Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4011-6
pubmed: 16537477
Infection. 2020 Aug;48(4):631-634
pubmed: 32524515
Nat Biomed Eng. 2020 Dec;4(12):1180-1187
pubmed: 32948854
Diagn Microbiol Infect Dis. 2013 Aug;76(4):470-6
pubmed: 23726649
Eur J Clin Microbiol Infect Dis. 2021 Apr;40(4):751-759
pubmed: 33078221
Clin Immunol. 2004 Mar;110(3):252-66
pubmed: 15047203
Diagnostics (Basel). 2021 Mar 03;11(3):
pubmed: 33802453