The interaction between RPAP3 and TRBP reveals a possible involvement of the HSP90/R2TP chaperone complex in the regulation of miRNA activity.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
28 02 2022
28 02 2022
Historique:
accepted:
27
01
2022
revised:
21
01
2022
received:
25
02
2021
pubmed:
13
2
2022
medline:
16
4
2022
entrez:
12
2
2022
Statut:
ppublish
Résumé
MicroRNAs silence mRNAs by guiding the RISC complex. RISC assembly occurs following cleavage of pre-miRNAs by Dicer, assisted by TRBP or PACT, and the transfer of miRNAs to AGO proteins. The R2TP complex is an HSP90 co-chaperone involved in the assembly of ribonucleoprotein particles. Here, we show that the R2TP component RPAP3 binds TRBP but not PACT. The RPAP3-TPR1 domain interacts with the TRBP-dsRBD3, and the 1.5 Å resolution crystal structure of this complex identifies key residues involved in the interaction. Remarkably, binding of TRBP to RPAP3 or Dicer is mutually exclusive. Additionally, we found that AGO(1/2), TRBP and Dicer are all sensitive to HSP90 inhibition, and that TRBP sensitivity is increased in the absence of RPAP3. Finally, RPAP3 seems to impede miRNA activity, raising the possibility that the R2TP chaperone might sequester TRBP to regulate the miRNA pathway.
Identifiants
pubmed: 35150569
pii: 6527678
doi: 10.1093/nar/gkac086
pmc: PMC8887487
doi:
Substances chimiques
HSP90 Heat-Shock Proteins
0
MicroRNAs
0
Molecular Chaperones
0
Nuclear Receptor Coactivators
0
RNA-Induced Silencing Complex
0
Ribonuclease III
EC 3.1.26.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2172-2189Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
J Biomol NMR. 2009 Aug;44(4):213-23
pubmed: 19548092
Genes Dev. 2010 Sep 15;24(18):2019-30
pubmed: 20801936
Cell Rep. 2014 Nov 6;9(3):1061-74
pubmed: 25437560
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Nat Commun. 2021 Aug 10;12(1):4810
pubmed: 34376666
Mol Biol Cell. 2009 Jul;20(14):3273-84
pubmed: 19458189
Cell Rep. 2017 Mar 14;18(11):2635-2650
pubmed: 28297668
RNA Biol. 2012 Feb;9(2):148-54
pubmed: 22418846
RNA Biol. 2008 Apr-Jun;5(2):92-103
pubmed: 18421256
J Cell Biol. 2014 Nov 24;207(4):463-80
pubmed: 25404746
Cell. 2009 Feb 20;136(4):642-55
pubmed: 19239886
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Biochemistry. 2011 May 31;50(21):4550-60
pubmed: 21526770
Nucleic Acids Res. 2013 Jul;41(13):6568-76
pubmed: 23661684
Methods. 2009 Aug;48(4):381-6
pubmed: 19450687
Methods. 2019 Jul 15;164-165:73-80
pubmed: 31026591
Mol Cell. 2010 Jul 30;39(2):282-91
pubmed: 20605502
Science. 1991 Mar 29;251(5001):1597-600
pubmed: 2011739
Int J Mol Sci. 2019 Dec 07;20(24):
pubmed: 31817850
Biomol Concepts. 2014 Dec;5(6):513-20
pubmed: 25429602
Can J Gastroenterol Hepatol. 2018 Jun 19;2018:5769591
pubmed: 30018946
Nucleic Acids Res. 2014 Feb;42(3):2015-36
pubmed: 24234454
Mol Biol Cell. 2010 May 1;21(9):1462-9
pubmed: 20237157
J Cell Biol. 2017 Jun 5;216(6):1579-1596
pubmed: 28515276
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2184-95
pubmed: 15572771
Trends Biochem Sci. 2018 Jan;43(1):4-9
pubmed: 29203338
Nucleic Acids Res. 2020 Apr 17;48(7):3848-3868
pubmed: 31996908
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W349-57
pubmed: 23748958
Cell. 2018 May 17;173(5):1191-1203.e12
pubmed: 29706542
J Biol Chem. 2003 Feb 14;278(7):4440-8
pubmed: 12475984
J Struct Biol. 2011 Aug;175(2):178-88
pubmed: 21320604
Nat Commun. 2016 Dec 09;7:13694
pubmed: 27934859
RNA. 2012 Oct;18(10):1833-45
pubmed: 22923768
J Cell Biol. 2018 Jul 2;217(7):2583-2598
pubmed: 29743191
Mol Biol Cell. 2013 Aug;24(15):2303-10
pubmed: 23741051
Nat Struct Mol Biol. 2013 Dec;20(12):1358-66
pubmed: 24270878
Structure. 2014 Jun 10;22(6):805-18
pubmed: 24794838
Biochim Biophys Acta. 2012 Mar;1823(3):674-82
pubmed: 21945180
Bioinformatics. 2005 Aug 15;21(16):3433-4
pubmed: 15955779
Mol Cell. 2010 Jul 30;39(2):292-9
pubmed: 20605501
Curr Genet. 2018 Oct;64(5):965-970
pubmed: 29470645
Sci Adv. 2019 May 01;5(5):eaaw1616
pubmed: 31049401
Biochem Biophys Res Commun. 2013 Jan 4;430(1):320-4
pubmed: 23159623
Nat Commun. 2018 Apr 16;9(1):1501
pubmed: 29662061
Mol Cell. 2013 Jan 10;49(1):172-85
pubmed: 23142078
Nucleic Acids Res. 2013 Apr;41(7):4241-52
pubmed: 23435228
J Cell Biol. 2008 Feb 11;180(3):579-95
pubmed: 18268104
Biomol NMR Assign. 2015 Apr;9(1):99-102
pubmed: 24668569
Nat Commun. 2017 May 31;8:15615
pubmed: 28561026
Sci Rep. 2018 Jan 18;8(1):1020
pubmed: 29348664
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):32-41
pubmed: 17164524
Mol Cell. 2010 Sep 24;39(6):912-924
pubmed: 20864038
Nat Struct Mol Biol. 2010 Aug;17(8):1024-6
pubmed: 20639883
Biol Open. 2020 Feb 25;9(2):
pubmed: 32051109
Methods Mol Biol. 2015;1296:187-98
pubmed: 25791601
Structure. 2018 Sep 4;26(9):1196-1209.e8
pubmed: 30033218
Nucleic Acids Res. 2017 Dec 1;45(21):12577-12584
pubmed: 29045748
J Virol. 2011 Dec;85(23):12614-21
pubmed: 21937648
Cell Rep. 2016 Apr 5;15(1):210-218
pubmed: 27052166
Structure. 2013 Oct 8;21(10):1834-47
pubmed: 24012479
Nature. 2019 Jan;565(7740):500-504
pubmed: 30626973
Mol Cell. 2010 Sep 24;39(6):839-50
pubmed: 20864032
Nucleic Acids Res. 2015 Oct 15;43(18):8973-89
pubmed: 26275778
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
Mol Cell. 2015 Feb 5;57(3):397-407
pubmed: 25557550
Nat Commun. 2018 May 29;9(1):2093
pubmed: 29844425
Cell Rep. 2014 Apr 10;7(1):19-26
pubmed: 24656813
Science. 2005 Sep 2;309(5740):1573-6
pubmed: 16081698
BMC Mol Biol. 2009 May 07;10:38
pubmed: 19422693
Jpn Dent Sci Rev. 2020 Dec;56(1):38-42
pubmed: 31890057