Apigenin induces oxidative stress in mouse Sertoli TM4 cells.
TM4 cells
apigenin
malondialdehyde
oxidative stress
reactive oxygen species
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
04
08
2021
accepted:
02
11
2021
entrez:
14
2
2022
pubmed:
15
2
2022
medline:
15
2
2022
Statut:
ppublish
Résumé
Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells. Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays. Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells. Exposure to API induced oxidative stress in mouse Sertoli TM4 cells.
Sections du résumé
BACKGROUND AND AIM
OBJECTIVE
Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells.
MATERIALS AND METHODS
METHODS
Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays.
RESULTS
RESULTS
Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells.
CONCLUSION
CONCLUSIONS
Exposure to API induced oxidative stress in mouse Sertoli TM4 cells.
Identifiants
pubmed: 35153403
doi: 10.14202/vetworld.2021.3132-3137
pii: Vetworld-14-3132
pmc: PMC8829396
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3132-3137Informations de copyright
Copyright: © Jittapalapong, et al.
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