Comparison of enzyme-linked immunosorbent assay and Fassisi
antibody concentration
calves
colostrum
passive transfer
rapid method
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
03
06
2021
accepted:
08
11
2021
entrez:
14
2
2022
pubmed:
15
2
2022
medline:
15
2
2022
Statut:
ppublish
Résumé
Rapid tests are routinely used to estimate serum immunoglobulin G (IgG) concentrations in diagnosing a failure of passive transfer (FPT) in calves. The study aimed to compare the Fassisi A total of 277 calves of 1-10 days of age were used in this study. Blood samples were obtained, and serum was extracted by centrifuging the samples at 2740× The mean ELISA-IgG serum concentration was 8.40 mg/mL (SD=7.02, range=0.10-47.50 mg/mL). FPT prevalence based on the ELISA measurements was 66.8%. The prevalence of partial and full FPT based on the FB-IgG was 54.5%. The ELISA-IgG and FB-IgG results were subjected to correlation and regression analysis. Overall sensitivity and specificity of the FB-IgG were 61.1% and 58.7%, respectively. A statistically significant dependence on age was identified in the results. Our findings suggest that the FB-IgG rapid method is less accurate and provides no other advantages over established methods.
Sections du résumé
BACKGROUND AND AIM
OBJECTIVE
Rapid tests are routinely used to estimate serum immunoglobulin G (IgG) concentrations in diagnosing a failure of passive transfer (FPT) in calves. The study aimed to compare the Fassisi
MATERIALS AND METHODS
METHODS
A total of 277 calves of 1-10 days of age were used in this study. Blood samples were obtained, and serum was extracted by centrifuging the samples at 2740×
RESULTS
RESULTS
The mean ELISA-IgG serum concentration was 8.40 mg/mL (SD=7.02, range=0.10-47.50 mg/mL). FPT prevalence based on the ELISA measurements was 66.8%. The prevalence of partial and full FPT based on the FB-IgG was 54.5%. The ELISA-IgG and FB-IgG results were subjected to correlation and regression analysis. Overall sensitivity and specificity of the FB-IgG were 61.1% and 58.7%, respectively. A statistically significant dependence on age was identified in the results.
CONCLUSION
CONCLUSIONS
Our findings suggest that the FB-IgG rapid method is less accurate and provides no other advantages over established methods.
Identifiants
pubmed: 35153414
doi: 10.14202/vetworld.2021.3211-3215
pii: Vetworld-14-3211
pmc: PMC8829403
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3211-3215Informations de copyright
Copyright: © Hampe, et al.
Références
J Vet Intern Med. 2000 Nov-Dec;14(6):569-77
pubmed: 11110376
Am J Vet Res. 2002 Feb;63(2):247-50
pubmed: 11843125
J Dairy Sci. 2020 Aug;103(8):7611-7624
pubmed: 32448583
Ir Vet J. 2015 Aug 25;68(1):18
pubmed: 26309724
J Dairy Sci. 2018 Oct;101(10):9168-9184
pubmed: 29908815
J Dairy Sci. 2014;97(6):3838-44
pubmed: 24704239
Animals (Basel). 2021 Mar 11;11(3):
pubmed: 33799858
J Am Vet Med Assoc. 2002 Mar 15;220(6):791-3
pubmed: 11918273
J Dairy Sci. 2018 Apr;101(4):3099-3109
pubmed: 29397179
Z Ernahrungswiss. 1995 Jun;34(2):160-3
pubmed: 8525649
Vet Clin North Am Food Anim Pract. 2019 Nov;35(3):535-556
pubmed: 31590901