Co-flow injection for serial crystallography at X-ray free-electron lasers.
3D printing
X-ray free-electron lasers
XFELs
microfluidic devices
sample consumption
serial crystallography
viscous media
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Feb 2022
01 Feb 2022
Historique:
received:
14
05
2021
accepted:
22
10
2021
entrez:
14
2
2022
pubmed:
15
2
2022
medline:
15
2
2022
Statut:
epublish
Résumé
Serial femtosecond crystallography (SFX) is a powerful technique that exploits X-ray free-electron lasers to determine the structure of macro-molecules at room temperature. Despite the impressive exposition of structural details with this novel crystallographic approach, the methods currently available to introduce crystals into the path of the X-ray beam sometimes exhibit serious drawbacks. Samples requiring liquid injection of crystal slurries consume large quantities of crystals (at times up to a gram of protein per data set), may not be compatible with vacuum configurations on beamlines or provide a high background due to additional sheathing liquids present during the injection. Proposed and characterized here is the use of an immiscible inert oil phase to supplement the flow of sample in a hybrid microfluidic 3D-printed co-flow device. Co-flow generation is reported with sample and oil phases flowing in parallel, resulting in stable injection conditions for two different resin materials experimentally. A numerical model is presented that adequately predicts these flow-rate conditions. The co-flow generating devices reduce crystal clogging effects, have the potential to conserve protein crystal samples up to 95% and will allow degradation-free light-induced time-resolved SFX.
Identifiants
pubmed: 35153640
doi: 10.1107/S1600576721011079
pii: S1600576721011079
pmc: PMC8805165
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1-13Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM095583
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM117342
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM120537
Pays : United States
Organisme : NIH HHS
ID : S10 OD021816
Pays : United States
Informations de copyright
© Diandra Doppler et al. 2022.
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