Cyclooxygenase 2 Effector Genes as Potential Inflammation-Related Biomarkers for Colorectal Cancer Circulating Tumor Cells Detection by Liquid Biopsy.

Parsortix system circulating tumor cells colorectal cancer cyclooxygenase 2-regulated genes inflammation-related genes liquid biopsy

Journal

Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923

Informations de publication

Date de publication:
2021
Historique:
received: 31 10 2021
accepted: 21 12 2021
entrez: 14 2 2022
pubmed: 15 2 2022
medline: 15 2 2022
Statut: epublish

Résumé

Cyclooxygenase 2 (COX2) has been implicated in cancer development and metastasis. We have identified several COX2-regulated inflammation-related genes in human colorectal cancer cells and shown that some of them play important roles in tumor progression. In this work, we have studied the COX2-regulated genes in the mouse colorectal cancer cell line CT26, to find that many are also regulated by COX2 over-expression. On the other hand, we generated a CT26 cell line expressing Gfp and Luciferase, to study tumor growth and metastasis in immunocompetent Balb/c mice. We then collected solid tissue, and blood samples, from healthy and tumor-bearing mice. Using the Parsortix

Identifiants

pubmed: 35153760
doi: 10.3389/fphar.2021.806395
pii: 806395
pmc: PMC8831911
doi:

Types de publication

Journal Article

Langues

eng

Pagination

806395

Informations de copyright

Copyright © 2022 Stamatakis, Torres-Gérica, Jiménez-Segovia, Ramos-Muñoz, Crespo-Toro, Fuentes, Toribio, Callejas-Hernández, Carrato, García Bermejo and Fresno.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Konstantinos Stamatakis (K)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.
Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Patricia Torres-Gérica (P)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.
Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Alba Jiménez-Segovia (A)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.
Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Edurne Ramos-Muñoz (E)

Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain.

Lorena Crespo-Toro (L)

Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain.

Patricia Fuentes (P)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.

María L Toribio (ML)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.

Francisco Callejas-Hernández (F)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.
Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Alfredo Carrato (A)

Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain.

María Laura García Bermejo (ML)

Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain.

Manuel Fresno (M)

Centro de Biología Molecular Severo Ochoa, UAM/CSIC, Madrid, Spain.
Department of Molecular Biology, Universidad Autónoma de Madrid, Madrid, Spain.

Classifications MeSH